High-Performance Liquid Chromatography-Fluorescence Detection Method for Ochratoxin A Quantification in Small Mice...

Area of Science:

• Analytical Chemistry
• Toxicology
• Neuroscience

Background:

• Ochratoxin A (OTA) is a prevalent mycotoxin with potential neurotoxic effects.
• Existing analytical methods lack validation for key tissues in mouse models of neurodegeneration.
• Accurate quantification of OTA in brain and intestine is critical for understanding its health risks.

Purpose of the Study:

• To develop and validate a sensitive HPLC-FLD method for OTA quantification.
• To include critical tissues like the brain and intestine in the validation process.
• To provide a reliable tool for neurodegeneration research involving OTA.

Main Methods:

• High-Performance Liquid Chromatography with Fluorescence Detection (HPLC-FLD) was employed.
• Method validation included selectivity, linearity, accuracy, precision, recovery, and stability.
• Analysis was performed on mouse plasma, kidney, liver, brain, and intestine samples.

Main Results:

• The developed HPLC-FLD method demonstrated high sensitivity, accuracy, and precision.
• All validation parameters met FDA and EMA guidelines.
• The method successfully quantified OTA in minimal tissue masses with excellent recovery.

Conclusions:

• A robust and validated HPLC-FLD method for OTA determination in mice tissues and plasma is now available.
• This method is suitable for neurodegeneration research, enabling accurate OTA quantification in relevant biological matrices.
• The validated method supports further investigation into the neurotoxicological effects of Ochratoxin A.