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Updated: Jun 25, 2025
07:16
Monitoring Hippo Signaling Pathway Activity Using a Luciferase-based Large Tumor Suppressor LATS Biosensor
Published on: September 13, 2018
19.8K
PAX8-AS1/microRNA-25-3p/LATS2 regulates malignant progression of ovarian cancer via Hippo signaling
1Department of Gynaecology, University-Town Hospital of Chongqing Medical University, Chongqing 400000, China.
Mutation Research
|May 24, 2024
Summary
This study reveals that PAX8-AS1, microRNA-25-3p, and LATS2 regulate ovarian cancer (OC) progression via the Hippo signaling pathway. Targeting this axis offers a potential new therapeutic strategy for OC treatment.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Ovarian cancer (OC) is a prevalent female reproductive malignancy.
- Aberrant long non-coding RNA (lncRNA) expression is implicated in cancer development.
- The role of PAX8-AS1 in OC progression is currently unclear.
Purpose of the Study:
- To investigate the role and molecular mechanism of PAX8-AS1 in the malignant progression of OC.
- To elucidate the regulatory network involving PAX8-AS1, microRNA-25-3p, and LATS2 in OC.
Main Methods:
- Bioinformatic analysis to assess gene expression and binding sites.
- Quantitative real-time PCR (qRT-PCR) for gene expression analysis.
- Cellular assays (CCK-8, colony formation, scratch healing, Transwell) to evaluate malignant behaviors.
- Fluorescence in situ hybridization (FISH) for subcellular localization.
- Western blot and immunofluorescence for protein expression and translocation.
- Dual luciferase assays to validate molecular interactions.
Main Results:
- PAX8-AS1 and LATS2 were downregulated, while microRNA-25-3p was upregulated in OC.
- PAX8-AS1 acts as a sponge for microRNA-25-3p, regulating LATS2 expression.
- Overexpression of PAX8-AS1 suppressed OC cell malignancy, an effect reversed by microRNA-mimic.
- LATS2 modulated YAP/TAZ phosphorylation and cytoplasmic translocation, impacting OC progression.
- Knockdown of PAX8-AS1 promoted OC progression, which was counteracted by LATS2 overexpression.
Conclusions:
- The PAX8-AS1/microRNA-25-3p/LATS2 axis regulates OC malignant progression through the Hippo signaling pathway.
- This axis represents a potential novel therapeutic target for ovarian cancer treatment.
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