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Related Experiment Videos

Circulating immune complexes and rheumatoid arthritis.

P Faaber, T P Rijke Schilder, P J Capel

    The Journal of Rheumatology
    |October 1, 1985
    PubMed
    Summary

    The Clq binding assay (ClqBA) may overestimate circulating immune complexes (CIC) in rheumatoid arthritis (RA) due to in vitro formation of RF IgM IgG complexes induced by polyethylene glycol (PEG). These complexes do not reflect in vivo conditions.

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    Nederlands tijdschrift voor geneeskunde·1999

    Area of Science:

    • Immunology
    • Rheumatology
    • Biochemistry

    Background:

    • Circulating immune complexes (CIC) are implicated in rheumatoid arthritis (RA) pathogenesis.
    • The Clq binding assay (ClqBA) is commonly used to detect CIC in RA patients.

    Purpose of the Study:

    • To investigate the discrepancy between ClqBA results and ultracentrifugation findings for CIC in RA.
    • To elucidate the role of polyethylene glycol (PEG) in CIC detection by ClqBA.

    Main Methods:

    • Comparison of ClqBA with ultracentrifugation on sucrose gradients.
    • Investigation of PEG's effect on rheumatoid factor (RF) IgM and IgG interactions.
    • Use of enzyme-linked immunosorbent assay (ELISA) to assess complex formation.

    Main Results:

    • CIC detected by ClqBA in RA sera were not confirmed by ultracentrifugation.
    • Polyethylene glycol (PEG) enhanced the avidity of RF, promoting in vitro formation of RF IgM IgG Clq complexes.
    • These PEG-induced complexes dissociated upon PEG removal, indicating they were not representative of in vivo CIC.

    Conclusions:

    • The ClqBA may yield false positives for CIC in RA due to PEG-induced in vitro complex formation.
    • RF IgM and IgG interactions are significantly influenced by PEG, affecting assay results.
    • ClqBA results for CIC in RA may not accurately reflect the in vivo immunological status.

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