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Expanding the horizons of levan: from microbial biosynthesis to applications and advanced detection methods
Sijie Wang1, Bo Wu2, Tatsaporn Todhanakasem3
1School of Food Industry, King Mongkut's Institute of Technology Ladkrabang, Bangkok, 10520, Thailand.
Abstract:
Levan, a β-(2,6)-linked fructose polymer, exhibits diverse properties that impart versatility, rendering it a highly sought-after biopolymer with various industrial applications. Levan can be produced by various microorganisms using sucrose, food industry byproducts and agricultural wastes. Microbial levan represents the most potent cost-effective process for commercial-scale levan production. This study reviews the optimization of levan production by understanding its biosynthesis, physicochemical properties and the fermentation process. In addition, genetic and protein engineering for its increased production and emerging methods for its detection are introduced and discussed. All of these comprehensive studies could serve as powerful tools to optimize levan production and broaden its applications across various industries.
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