Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Cytotoxic T Cells-mediated Immune Response

Cytotoxic T Cells-mediated Immune Response

Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Boronic Acid-Functionalized MOFs Enable Ultrasensitive Proteomic Profiling of Ultratrace Plasma for Triple-Negative Breast Cancer Diagnosis.

Analytical chemistry·2026

Same author

Machine Learning-Driven Optimization of Viscoelastic Microfluidic Particle Separation.

Analytical chemistry·2026

Same author

Robotic acoustofluidic single-cell picking and placement platform.

Lab on a chip·2026

Same author

Preeclampsia Diagnosis Using β-Hairpin Peptide for ELABELA Detection.

Journal of peptide science : an official publication of the European Peptide Society·2026

Same author

NSD2 inhibits the expression of PD-L1 via oxidative phosphorylation to control immune surveillance in hepatocellular carcinoma.

Cell death & disease·2026

Same author

Histone 3 lysine 36 trimethylation by SETD2 shapes an epigenetic landscape in intestinal stem cells to orchestrate lipid metabolism and attenuate cell senescence.

Cell death & disease·2026

Search research articles

Related Experiment Video

Updated: Jun 21, 2026

Isolation and Characterization of Neutrophils with Anti-Tumor Properties

Isolation and Characterization of Neutrophils with Anti-Tumor Properties

Published on: June 19, 2015

Region-Specific CD16⁺ Neutrophils Promote Colorectal Cancer Progression by Inhibiting Natural Killer Cells.

Yan Zhang^1,2, Zien Wang^1,2, Yu Lu¹

¹State Key Laboratory of Systems Medicine for Cancer, Renji Hospital, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200030, China.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)

|May 25, 2024

Summary

Researchers discovered CD16+ neutrophils in colorectal cancer (CRC) tumors that promote cancer by harming natural killer (NK) cells. Targeting these neutrophils could offer new CRC diagnostics and therapies.

Keywords:

CRC NET NK cell cholesterol neutrophil

More Related Videos

Neutrophil Isolation and Analysis to Determine their Role in Lymphoma Cell Sensitivity to Therapeutic Agents

Neutrophil Isolation and Analysis to Determine their Role in Lymphoma Cell Sensitivity to Therapeutic Agents

Published on: March 25, 2016

Transfer of Manipulated Tumor-associated Neutrophils into Tumor-Bearing Mice to Study their Angiogenic Potential In Vivo

Transfer of Manipulated Tumor-associated Neutrophils into Tumor-Bearing Mice to Study their Angiogenic Potential In Vivo

Published on: July 20, 2019

Related Experiment Videos

Last Updated: Jun 21, 2026

Isolation and Characterization of Neutrophils with Anti-Tumor Properties

Isolation and Characterization of Neutrophils with Anti-Tumor Properties

Published on: June 19, 2015

Neutrophil Isolation and Analysis to Determine their Role in Lymphoma Cell Sensitivity to Therapeutic Agents

Neutrophil Isolation and Analysis to Determine their Role in Lymphoma Cell Sensitivity to Therapeutic Agents

Published on: March 25, 2016

Transfer of Manipulated Tumor-associated Neutrophils into Tumor-Bearing Mice to Study their Angiogenic Potential In Vivo

Transfer of Manipulated Tumor-associated Neutrophils into Tumor-Bearing Mice to Study their Angiogenic Potential In Vivo

Published on: July 20, 2019

Area of Science:

Immunology
Oncology
Molecular Biology

Background:

The colon hosts a significant immune presence, yet innate immune responses in colorectal cancer (CRC) are not fully understood.
Understanding how innate immune cells are activated is crucial for developing effective CRC treatments.

Purpose of the Study:

To identify and characterize innate immune cells involved in colorectal cancer progression.
To elucidate the mechanisms by which these cells influence the tumor microenvironment and impact anti-tumor immunity.

Main Methods:

Utilized imaging mass cytometry (IMC), immunofluorescence, and flow cytometry to identify specific neutrophil populations in CRC tissues.
Investigated the molecular pathways, including the CD16/TAK1/NF-κB axis, involved in neutrophil function and cholesterol metabolism.
Assessed the impact of CD16+ neutrophils on natural killer (NK) cell activity and tumor progression.

Main Results:

Identified a distinct population of CD16+ neutrophils that accumulate in CRC tumors and exhibit pro-tumor activity.
Found that these neutrophils have abnormal cholesterol accumulation, driven by the CD16/TAK1/NF-κB pathway, upregulating scavenger receptors (CD36, LRP1).
Demonstrated that CD16+ neutrophils impair NK cell function by inhibiting cholesterol uptake, disrupting lipid raft formation, and inducing NK cell death via neutrophil extracellular traps (NETs).

Conclusions:

CRC-associated CD16+ neutrophils possess pro-tumor functions by suppressing NK cell-mediated anti-tumor immunity.
These neutrophils represent a potential diagnostic biomarker for CRC.
Targeting CD16+ neutrophils offers a promising therapeutic strategy for colorectal cancer treatment.