Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Complex Assembly02:41

Protein Complex Assembly

Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Protein Complex Assembly02:41

Protein Complex Assembly

Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Enhancing skin-implant integration in lower-limb transcutaneous prostheses: From interface biology to bioactive, antimicrobial and cell-based strategies.

Journal of orthopaedic translation·2026
Same author

Simple fabrication method for cancer cell migration studies on biomimetic substrates with tunable stiffness.

Materials today. Bio·2026
Same author

Thermally Degradable Biocompatible Hydrogel as Transient Encapsulation Coating for Implantable Sensors.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Albumin coating of magnetic nanoparticles for imaging, tracking and delivery through biological barriers.

Nanoscale·2026
Same author

My cells, my model: immune-competent autologous organ-on-chip systems as a new paradigm in precision medicine.

Frontiers in immunology·2025
Same author

Morpho-Functional Responsiveness of Caco-2 Enterocyte-like Monolayers to Insulin in a Pro-Inflammatory Environment.

Cells·2025

Related Experiment Video

Updated: May 7, 2026

Development of Amelogenin-chitosan Hydrogel for In Vitro Enamel Regrowth with a Dense Interface
08:26

Development of Amelogenin-chitosan Hydrogel for In Vitro Enamel Regrowth with a Dense Interface

Published on: July 10, 2014

15.0K

Towards Complex Tissues Replication: Multilayer Scaffold Integrating Biomimetic Nanohydroxyapatite/Chitosan

Barbara Palazzo1, Stefania Scialla2, Amilcare Barca3

  • 1ENEA, Division for Sustainable Materials, Brindisi Research Center, S.S. 7 Appia Km. 706, 72100 Brindisi, Italy.

Bioengineering (Basel, Switzerland)
|May 25, 2024
PubMed
Summary

Researchers developed a novel multilayer scaffold using chitosan and nanohydroxyapatite (Cs/n-HAp) via biomineralization. This biomaterial mimics natural tissues and shows promising cytocompatibility for tissue engineering applications.

Keywords:
biomineralizationchitosanhydroxyapatitemultilayered scaffolds

More Related Videos

3D Printed Porous Cellulose Nanocomposite Hydrogel Scaffolds
06:36

3D Printed Porous Cellulose Nanocomposite Hydrogel Scaffolds

Published on: April 24, 2019

9.6K
Synthesis of Graphene-Hydroxyapatite Nanocomposites for Potential Use in Bone Tissue Engineering
07:14

Synthesis of Graphene-Hydroxyapatite Nanocomposites for Potential Use in Bone Tissue Engineering

Published on: July 27, 2022

3.7K

Related Experiment Videos

Last Updated: May 7, 2026

Development of Amelogenin-chitosan Hydrogel for In Vitro Enamel Regrowth with a Dense Interface
08:26

Development of Amelogenin-chitosan Hydrogel for In Vitro Enamel Regrowth with a Dense Interface

Published on: July 10, 2014

15.0K
3D Printed Porous Cellulose Nanocomposite Hydrogel Scaffolds
06:36

3D Printed Porous Cellulose Nanocomposite Hydrogel Scaffolds

Published on: April 24, 2019

9.6K
Synthesis of Graphene-Hydroxyapatite Nanocomposites for Potential Use in Bone Tissue Engineering
07:14

Synthesis of Graphene-Hydroxyapatite Nanocomposites for Potential Use in Bone Tissue Engineering

Published on: July 27, 2022

3.7K

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Nanotechnology

Background:

  • Natural tissues possess complex, layered structures crucial for function.
  • Developing synthetic scaffolds that mimic these intricate architectures is a key challenge in regenerative medicine.
  • Existing scaffolds often lack the precise control needed to replicate the graded composition of natural tissues.

Purpose of the Study:

  • To design and prepare a multilayer scaffold that mimics the interstratified structure of natural tissues.
  • To investigate the use of in situ biomineralization for creating graded chitosan/nanohydroxyapatite (Cs/n-HAp) constructs.
  • To evaluate the structural, mechanical, and cytocompatibility properties of the developed scaffolds.

Main Methods:

  • Fabrication of multilayer scaffolds using chitosan matrices with varying nanohydroxyapatite (n-HAp) concentrations (10%, 20%, 30% wt%) via in situ biomineralization.
  • Utilized a freeze-drying technique for scaffold preparation.
  • Assessed scaffold properties including n-HAp nucleation, distribution, mechanical strength, and cytocompatibility using cell proliferation and apoptosis assays.

Main Results:

  • Achieved successful Cs/n-HAp scaffold fabrication with controlled n-HAp nucleation and homogeneous distribution.
  • Demonstrated improved mechanical properties in the Cs/n-HAp scaffolds compared to pure chitosan.
  • Exhibited good cytocompatibility, with cell proliferation similar to controls at 10% n-HAp and no induced apoptosis up to 20% n-HAp.

Conclusions:

  • The in situ biomineralization approach enables the creation of multilayered Cs/n-HAp scaffolds with graded compositions.
  • The developed scaffolds show potential for mimicking complex natural tissues, such as osteochondral tissue.
  • This strategy offers a promising route for advanced tissue engineering applications requiring biomimetic multilayered constructs.