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Related Experiment Video

Updated: Jun 20, 2026

Glutamine Flux Imaging Using Genetically Encoded Sensors

Glutamine Flux Imaging Using Genetically Encoded Sensors

Published on: July 31, 2014

Engineering the Signal Resolution of a Paper-Based Cell-Free Glutamine Biosensor with Genetic Engineering, Metabolic

Tyler J Free¹, Joseph P Talley¹, Chad D Hyer¹

¹Department of Chemical Engineering, Brigham Young University, Provo, UT 84602, USA.

Sensors (Basel, Switzerland)

|May 25, 2024

Summary

Researchers developed a low-cost, paper-based glutamine biosensor for cancer treatment monitoring. This portable diagnostic offers improved signal resolution, enabling personalized medicine and overcoming limitations of current lab tests.

Keywords:

biosensor cancer cell-free protein synthesis (CFPS)glutamine

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Last Updated: Jun 20, 2026

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Published on: June 14, 2021

Area of Science:

Biomedical Engineering
Cancer Diagnostics
Biosensor Technology

Background:

Cancer treatments can leverage glutamine dependence for improved survival.
Current glutamine diagnostics are lab-based, expensive, and not widely accessible for frequent patient monitoring.
Personalized treatment requires accessible diagnostics to balance efficacy and side effects.

Purpose of the Study:

To engineer a low-cost, portable glutamine biosensor.
To overcome background and signal-to-noise limitations in previous glutamine detection methods.
To develop a diagnostic tool for personalized cancer treatment monitoring.

Main Methods:

Development of a cell-free glutamine biosensor.
Engineering a paper-based, colorimetric assay.
Optimization to enhance signal strength and signal-to-background ratio.

Main Results:

Successful engineering of a shelf-stable, paper-based, colorimetric glutamine test.
Achieved high signal strength and a high signal-to-background ratio.
Demonstrated improved signal resolution compared to previous studies.

Conclusions:

The developed glutamine test represents significant progress in cancer management and personalized medicine.
The cell-free biosensing platform is versatile for low-cost detection of various clinically relevant molecules.
This technology facilitates more accessible and frequent monitoring of patient response to cancer therapies.