Lymphocyte subpopulations transformation studies in an experimental model of intestinal and hepatic amoebiasis

The lymphocyte subpopulations in peripheral blood and blast transformation were studied on days 0, 3, 7, 11, 15, 20 and 35 after infection in progesterone-treated guinea-pigs which had been experimentally infected with Entamoeba histolytica by intracaecal inoculation. In animals with intestinal infection, lowered T cell numbers and blast transformation were seen during the acute phase with a subsequent tendency to recover. In guinea-pigs with hepatic amoebiasis, both these parameters were significantly depressed throughout the period of study with no tendency to recovery. In progesterone-treated and sham-operated animals, a less marked depression of T cells, was observed. The B cell population did not vary greatly in either group. This study has thus shown that in hepatic amoebiasis there is highly significant depression in both number and function of T cells in comparison to intestinal amoebiasis.