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The complete sequence of Bacillus phage phi 29 gene 16: a protein required for the genome encapsidation reaction
Abstract:
We have sequenced the region of the Bacillus phage phi 29 genome that encodes gene 16, the gene product of which catalyzes the in vivo and in vitro genome-encapsidation reaction. The identity of the coding frame was confirmed by sequencing a sus mutant, sus16(300). It is concluded that gene 16 encodes a 39-kDal protein and is comprised of 331 amino acids. Only 30 bp separates gene 16 from the last open reading frame of the right early region. Analysis of potential secondary structures in this region suggests that the same sequences may be involved in the termination of both the late and early transcripts.
Insights
Researchers sequenced the Bacillus phage phi 29 genome
Area of Science:
- Molecular Biology
- Genomics
- Virology
Background:
- The Bacillus phage phi 29 genome contains essential genes for viral replication.
- Gene 16 product is crucial for catalyzing genome encapsidation.
Purpose of the Study:
- To sequence and characterize the Bacillus phage phi 29 gene 16.
- To confirm the coding frame and protein product of gene 16.
Main Methods:
- DNA sequencing of the Bacillus phage phi 29 genome region encoding gene 16.
- Analysis of a sus mutant (sus16(300)) to confirm the coding frame.
Main Results:
- Gene 16 was identified and sequenced.
- Gene 16 encodes a 39-kDa protein of 331 amino acids.
- A short intergenic region (30 bp) separates gene 16 from the preceding open reading frame.
Conclusions:
- Gene 16 product's role in genome encapsidation is confirmed.
- Potential shared regulatory sequences for early and late gene transcription termination were identified.