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Related Experiment Videos

Improved assay for total hemolytic complement.

R J Clark, A R Tschida, H Markowitz

    Journal of Clinical & Laboratory Immunology
    |December 1, 1985
    PubMed
    Summary
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    A new semi-automated system simplifies total hemolytic complement (CH50) testing for autoimmune diseases. This cost-effective method accurately measures CH50 levels in human serum using an Apple IIe microprocessor and automated sample handling.

    Area of Science:

    • Clinical Chemistry
    • Immunology
    • Medical Diagnostics

    Background:

    • Total hemolytic complement (CH50) analysis is crucial for monitoring autoimmune diseases like lupus nephritis.
    • Existing methods can be labor-intensive and costly.
    • A need exists for more efficient and economical CH50 testing.

    Purpose of the Study:

    • To develop and validate a semi-automated system for CH50 analysis.
    • To improve the efficiency and reduce the cost of CH50 testing.
    • To provide a reliable method for monitoring patients with complement-related diseases.

    Main Methods:

    • Utilized an Autoprep dilutor controlled by an Apple IIe microprocessor for sample handling and dilution.
    • Employed a 96-well microplate format with sensitized sheep red blood cells (RBCs).

    Related Experiment Videos

  • Automated data processing using the von Krogh equation and a Dynatech MR600 plate reader.

    • Main Results:

    • The semi-automated system demonstrated efficient sample processing and dilution.
    • Microprocessor-driven calculations accurately determined percentage hemolysis and CH50 values.
    • Results for normal human serum samples showed good correlation with an established semi-automated method.

    Conclusions:

    • The developed semi-automated system offers a practical and cost-effective approach to CH50 testing.
    • This method facilitates accurate monitoring of patients with autoimmune and glomerulonephritis conditions.
    • The system streamlines laboratory workflow for complement analysis.