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miR-181a/b-5p negatively regulates keratinocytes proliferation by targeting MELK

Mutian Niu1,2, Mingzhao Li1,2, Xiaomei Fan1,2

  • 1School of Intelligent Medicine and Biotechnology, Guilin Medical University, Guilin, 541199, Guangxi, P. R. China.

Archives of Dermatological Research
|May 25, 2024
PubMed

Insights

MicroRNAs miR-181a-5p and miR-181b-5p are downregulated in psoriasis and inhibit keratinocyte proliferation. These miRNAs target MELK, suggesting potential diagnostic and therapeutic roles in psoriasis.

Area of Science:

  • Dermatology
  • Molecular Biology
  • Biochemistry

Background:

  • Psoriasis pathogenesis involves microRNAs (miRNAs).
  • Aberrant keratinocyte proliferation is a hallmark of psoriasis.
  • The specific roles of miR-181a-5p and miR-181b-5p in keratinocytes are not fully understood.

Purpose of the Study:

  • To investigate the involvement of miR-181a-5p and miR-181b-5p in HaCaT keratinocyte proliferation.
  • To elucidate the regulatory relationship between these miRNAs and Maternal Embryonic Leucine Zipper Kinase (MELK).

Main Methods:

  • Cell viability and proliferation assessed using CCK-8 and EdU assays.
  • Quantitative real-time PCR (qRT-PCR) and Western blotting for MELK and KRT16 expression.
  • Luciferase reporter assay to confirm miRNA-target interaction.

Main Results:

  • miR-181a-5p and miR-181b-5p expression was downregulated in psoriasis lesions.
  • These miRNAs negatively regulated HaCaT keratinocyte proliferation.
  • MELK was identified as a direct target of miR-181a-5p/miR-181b-5p.
  • MELK knockdown inhibited proliferation, while overexpression promoted it; miRNA mimics reversed MELK's effects.

Conclusions:

  • miR-181a-5p and miR-181b-5p negatively regulate keratinocyte proliferation by targeting MELK.
  • These miRNAs represent potential diagnostic biomarkers for psoriasis.
  • miR-181a-5p and miR-181b-5p offer potential therapeutic targets for psoriasis treatment.
Keywords:
KeratinocytesMELKProliferationmiR-181a-5pmiR-181b-5p

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