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Analytical control of imatinib in bioanalytical samples using graphene quantum dots sensing
María A García-Trejo1,2, Gregorio Castañeda1,2, Ángel Ríos3,4
1Department of Analytical Chemistry and Food Technology, Faculty of Chemical Sciences and Technologies, University of Castilla-La Mancha, Campus Universitario, 13071, Ciudad Real, Spain.
This study presents a new fluorescence-based method using graphene quantum dots (GQDs) to quantify imatinib (IMA), a crucial drug for chronic myeloid leukemia. The method enables accurate therapeutic drug monitoring in biological and pharmaceutical samples.
Area of Science:
- Analytical Chemistry
- Materials Science
Background:
- Imatinib (IMA) is a vital anticancer drug for chronic myeloid leukemia (CML).
- Accurate therapeutic drug monitoring and dose control of IMA are essential for patient outcomes.
- Novel analytical methods are needed for precise IMA determination in complex samples.
Purpose of the Study:
- To develop and validate a novel fluorescence-based analytical method for imatinib determination.
- To utilize graphene quantum dots (GQDs) as a sensitive probe for imatinib quantification.
- To enable reliable analytical control of imatinib in biological and pharmaceutical samples.
Main Methods:
- Solid-phase extraction (SPE) using tC18 sorbent cartridges for sample preparation.
- Measurement of fluorescence quenching of graphene quantum dots (GQDs) upon interaction with imatinib.
- Quantification of imatinib in the range of 10-250 µg L⁻¹.
Main Results:
- The method demonstrated a limit of detection of 21 µg L⁻¹.
- High precision was achieved with a relative standard deviation of 1.5% for reproducibility.
- Satisfactory recovery rates (84-113%) were obtained for biological samples.
Conclusions:
- The developed GQD-based fluorescence method offers a sensitive and reliable approach for imatinib analysis.
- This method is suitable for therapeutic drug monitoring and quality control of imatinib in various sample types.
- The fluorescence quenching mechanism provides a robust platform for quantitative determination of anticancer drugs.
