Related Experiment Video
Updated: Jul 14, 2026
10:07
Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages
Published on: June 23, 2013
Protocol for extracting and isolating porcine bone-marrow-derived macrophages from ribs
Francesco Boschetto1, Chi Ma1, Michael Seungcheol Kang2
1Center of Excellence in Hip, Scottish Rite for Children, Dallas, TX 75219, USA; Department of Orthopedic Surgery, UT Southwestern Medical Center, Dallas, TX 75390, USA.
STAR Protocols
|May 25, 2024
Summary
This study details a protocol for isolating bone-marrow-derived macrophages (BMDMs) from pig ribs. These cells are crucial for studying inflammation and immune responses in osteonecrosis and osteoimmunology research.
Area of Science:
- Biomedical Research
- Immunology
- Osteoimmunology
Background:
- Pigs share anatomical and biological similarities with humans.
- Pigs are increasingly utilized in inflammation and immune studies.
- Osteonecrosis and osteoimmunology research benefits from porcine models.
Purpose of the Study:
- To present a detailed protocol for obtaining bone-marrow-derived macrophages (BMDMs) from pigs.
- To facilitate in vitro studies on cell phenotype in porcine models.
- To support research in osteonecrosis and osteoimmunology.
Main Methods:
- Rib extraction from porcine subjects.
- Bone marrow isolation via centrifugation.
- Processing for bone-marrow-derived macrophage (BMDM) acquisition.
- In vitro cell phenotype evaluation.
Main Results:
- A reproducible protocol for BMDM isolation from pig ribs was established.
- The protocol enables the acquisition of cells suitable for in vitro analysis.
- Methodology supports further investigation into porcine osteoimmunology.
Conclusions:
- The presented protocol provides a reliable method for generating BMDMs from pigs.
- This resource aids in advancing osteonecrosis and osteoimmunology research using porcine models.
- The study facilitates standardized in vitro immunological assessments in pigs.
