Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Helicases00:55

DNA Helicases

21.3K
DNA unwinding helicase enzymes are a type of motor protein. Motor proteins can translocate along filaments or polymers using energy generated from ATP hydrolysis. Helicases are involved in all the important cellular processes where DNA unwinding is required, such as DNA replication, repair, recombination, and transcription. They are present in all living organisms, but vary in their structure, function, and mechanism of action. For example, in prokaryotes, DnaB helicase binds and translocates...
21.3K
Replication in Eukaryotes01:29

Replication in Eukaryotes

13.7K
In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
13.7K
The DNA Replication Fork01:02

The DNA Replication Fork

35.9K
An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
35.9K
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

5.8K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
5.8K
Replication in Prokaryotes01:32

Replication in Prokaryotes

24.8K
DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...
24.8K
The Replisome03:01

The Replisome

33.4K
DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
33.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Loss of nsp14-exonuclease activity impairs the replication, proofreading, fitness, and pathogenesis of SARS-CoV-2.

mBio·2026
Same author

Mechanism and spectrum of inhibition of viral polymerases by 2'-deoxy-2'-β-fluoro-4'-azidocytidine or azvudine.

NAR molecular medicine·2025
Same author

MERS-CoV spike vaccine-induced N-terminal domain-specific antibodies are more protective than receptor binding domain-specific antibodies.

iScience·2025
Same author

The coronavirus nsp14 exoribonuclease interface with the cofactor nsp10 is essential for efficient virus replication and enzymatic activity.

Journal of virology·2025
Same author

An oral non-covalent non-peptidic inhibitor of SARS-CoV-2 Mpro ameliorates viral replication and pathogenesis in vivo.

Cell reports·2024
Same author

The coronavirus nsp14 exoribonuclease interface with the cofactor nsp10 is essential for efficient virus replication and enzymatic activity.

bioRxiv : the preprint server for biology·2024
Same journal

Vaccination with an HSV-1 virus containing CD-137L reduces herpetic corneal disease when used both prophylactically and therapeutically.

Virus research·2026
Same journal

Ondansetron exhibits potent antiviral activity against enterovirus 71 infection in vitro and in vivo.

Virus research·2026
Same journal

Case report: A case of HIV seroconversion in a high-risk individual after 35 years of resistance with a CCR5Δ32 heterozygous genotype.

Virus research·2026
Same journal

Viruses under mechanical force: Implications and applications.

Virus research·2026
Same journal

Mechanisms and therapeutic implications of galectins regulating Epstein-Barr virus infection.

Virus research·2026
Same journal

Functional SLC29A3/ENT3 drives autophagic clearance of intracellular viral particles.

Virus research·2026
See all related articles

Related Experiment Video

Updated: Jun 25, 2025

Author Spotlight: Unraveling the Dynamics of Eukaryotic DNA Replication Through Single-Molecule Visualization
07:37

Author Spotlight: Unraveling the Dynamics of Eukaryotic DNA Replication Through Single-Molecule Visualization

Published on: September 27, 2024

1.5K

The Coronavirus helicase in replication.

Samantha L Grimes1, Mark R Denison1

  • 1Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, TN 37232, USA.

Virus Research
|May 25, 2024
PubMed
Summary
This summary is machine-generated.

The coronavirus nonstructural protein 13 RNA helicase (nsp13-HEL) is vital for viral replication and a key target for antiviral drugs. Research into its functions may lead to new helicase inhibitors.

Keywords:
COVID-19CoronavirusNidoviralesRNA helicaseReplicaseReviewSARS-CoV-2Sarbecovirusnsp13

More Related Videos

Author Spotlight: Investigating the Motion Dynamics of the Eukaryotic Replisome Components at the Single-Molecule Level
10:11

Author Spotlight: Investigating the Motion Dynamics of the Eukaryotic Replisome Components at the Single-Molecule Level

Published on: July 26, 2024

1.0K
Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
08:53

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

327

Related Experiment Videos

Last Updated: Jun 25, 2025

Author Spotlight: Unraveling the Dynamics of Eukaryotic DNA Replication Through Single-Molecule Visualization
07:37

Author Spotlight: Unraveling the Dynamics of Eukaryotic DNA Replication Through Single-Molecule Visualization

Published on: September 27, 2024

1.5K
Author Spotlight: Investigating the Motion Dynamics of the Eukaryotic Replisome Components at the Single-Molecule Level
10:11

Author Spotlight: Investigating the Motion Dynamics of the Eukaryotic Replisome Components at the Single-Molecule Level

Published on: July 26, 2024

1.0K
Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
08:53

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

327

Area of Science:

  • Virology
  • Molecular Biology
  • Biochemistry

Background:

  • Coronaviruses utilize nonstructural protein 13 RNA helicase (nsp13-HEL) for replication.
  • nsp13-HEL possesses essential unwinding and nucleoside triphosphatase (NTPase) activities.
  • Enzymatic inactivation of nsp13-HEL is a critical target for antiviral development.

Purpose of the Study:

  • To review the reported and proposed functions of coronavirus nsp13-HEL in viral replication.
  • To highlight research areas for developing nsp13-HEL inhibitors.

Main Methods:

  • Literature review of genetic and biochemical studies.
  • Analysis of structurally-related helicases.
  • Exploration of nsp13-HEL's enzymatic functions beyond canonical activities.

Main Results:

  • nsp13-HEL is confirmed as essential for viral replication.
  • Evidence supports novel roles for nsp13-HEL in coronavirus biology.
  • Potential new hypotheses for nsp13-HEL's function in replication are presented.

Conclusions:

  • nsp13-HEL is a high-priority target for antiviral drug discovery.
  • Further research into nsp13-HEL's diverse functions is crucial.
  • Understanding nsp13-HEL mechanisms can guide the development of effective helicase inhibitors.