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Related Experiment Video

Updated: Jun 25, 2025

Fabrication of 3D Cardiac Microtissue Arrays using Human iPSC-Derived Cardiomyocytes, Cardiac Fibroblasts, and Endothelial Cells
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Engineered Cardiac Microtissue Biomanufacturing Using Human Induced Pluripotent Stem Cell Derived Epicardial Cells.

Kirk Butler1, Saif Ahmed1, Justin Jablonski2

  • 1Biomedical Engineering Department, Binghamton University, the State University of New York, Binghamton NY 13902.

Biorxiv : the Preprint Server for Biology
|May 27, 2024
PubMed
Summary
This summary is machine-generated.

Stem cell-derived epicardial cells are vital for 3D heart tissue models. Low-passage cells yield more robust tissues and promote cardiomyocyte function, offering potential for cardiac tissue engineering.

Keywords:
cardiomyocytesepicardiumheterotypic interactionshuman pluripotent stem cellstissue engineering

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Area of Science:

  • Cardiovascular Biology
  • Stem Cell Biology
  • Tissue Engineering

Background:

  • Epicardial cells are essential for human heart development and in vitro cardiac tissue models.
  • Induced pluripotent stem cells offer a renewable source for generating human epicardial cells.
  • Studying epicardium development and function is challenging due to accessibility limitations.

Approach:

  • Small molecule modulation of Wnt signaling was used to derive epicardial cells from induced pluripotent stem cells.
  • Characterization of cell populations included WT1 expression, morphology, and passaging effects.
  • 3D microtissue formation and co-culture with cardiomyocytes were assessed to evaluate functional properties.

Key Points:

  • A robust population of epicardial cells (approx. 87.7% WT1+) was generated.
  • Cell expansion in vitro showed limitations, with larger cell size and decreased cell number over passages.
  • Low-passage epicardial cells formed more robust 3D microtissues compared to high-passage cells.
  • Two distinct morphologic subpopulations were observed, including larger, migratory cells.
  • Epicardial cells enhanced cardiac tissue remodeling and migration in co-culture without impairing cardiomyocyte function.

Conclusions:

  • Stem cell-derived epicardial cells can be reliably generated and characterized.
  • Early-passage epicardial cells are optimal for fabricating consistent engineered cardiac tissues.
  • These findings advance the potential of stem cell-derived epicardial cells for cardiac modeling and biomanufacturing.