Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Animal Mitochondrial Genetics02:59

Animal Mitochondrial Genetics

7.6K
Among all the organelles in an animal cell, only mitochondria have their own independent genomes. Animal mitochondrial DNA is a double-stranded, closed-circular molecule with around 20,000 base pairs. Mitochondrial DNA is unique in that one of its two strands, the heavy, or H, -strand is guanine rich, whereas the complementary strand is cytosine rich and called the light, or L, -strand. Compared to nuclear DNA, mitochondrial DNA has a very low percentage of non-coding regions and is marked by...
7.6K
RNA Editing02:23

RNA Editing

9.0K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
9.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Validation and analysis of 12,000 AI-driven CAR-T designs in the <i>Bits to Binders</i> competitions.

bioRxiv : the preprint server for biology·2026
Same author

Rewiring STAT signaling from the cell surface with Trikine immunotherapeutics.

Science (New York, N.Y.)·2026
Same author

ercc6 deficient zebrafish exhibit UV and metronidazole sensitivity, increased oxygen consumption, and impaired hair cell mechanoelectrical transduction which can be restored by the superoxide dismutase mimetic MnTBAP.

Human molecular genetics·2026
Same author

Epigenome-wide association study of nuclear DNA methylation in relation to mitochondrial heteroplasmy.

Nature communications·2025
Same author

Efficient and reliable measles reprogramming platform for the generation of human iPSC.

bioRxiv : the preprint server for biology·2025
Same author

Non-Destructive Larval Genotyping of <i>Danio rerio</i> for Mitochondrial and Nuclear DNA Genetics.

bioRxiv : the preprint server for biology·2025

Related Experiment Video

Updated: Jun 25, 2025

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing
07:24

Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing

Published on: February 10, 2023

1.5K

Unconstrained Precision Mitochondrial Genome Editing with αDdCBEs.

Santiago R Castillo, Brandon W Simone, Karl J Clark

    Biorxiv : the Preprint Server for Biology
    |May 27, 2024
    PubMed
    Summary

    Researchers developed new base editors (αDdCBEs) that overcome limitations in editing mitochondrial DNA (mtDNA). These advanced editors allow precise C•G-to-T•A conversions at previously inaccessible mtDNA sites, improving therapeutic potential.

    More Related Videos

    Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e
    07:31

    Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e

    Published on: February 17, 2023

    1.1K
    CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
    07:46

    CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

    Published on: December 11, 2020

    5.8K

    Related Experiment Videos

    Last Updated: Jun 25, 2025

    Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing
    07:24

    Genotyping Single Nucleotide Polymorphisms in the Mitochondrial Genome by Pyrosequencing

    Published on: February 10, 2023

    1.5K
    Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e
    07:31

    Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e

    Published on: February 17, 2023

    1.1K
    CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
    07:46

    CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

    Published on: December 11, 2020

    5.8K

    Area of Science:

    • Mitochondrial genetics
    • Molecular biology
    • Gene editing technologies

    Background:

    • DddA-derived cytosine base editors (DdCBEs) target C•G-to-T•A conversions in mitochondrial DNA (mtDNA), crucial for understanding cellular processes and treating genetic disorders.
    • TALE-based DdCBEs face limitations due to a 5'-T constraint, restricting editing to over 150 human mtDNA loci.

    Approach:

    • Engineered modified TALE proteins in αDdCBEs to recognize all 5' bases, challenging the 5'-T constraint.
    • Assessed the activity and specificity of αDdCBEs compared to conventional DdCBEs across diverse mtDNA loci.
    • Validated the compatibility of αDdCBEs with DddA derivatives and TALE shifting for optimized base editing.

    Key Points:

    • αDdCBEs successfully edited mtDNA at diverse loci, irrespective of the 5'-most base.
    • αDdCBEs demonstrated superior activity and specificity compared to conventional DdCBEs.
    • The 5'-T constraint for TALE-based mtDNA editing was overcome, expanding editable sites.

    Conclusions:

    • αDdCBEs enable efficient, specific, and unconstrained base editing in mtDNA.
    • This technology broadens the scope of potential therapeutic applications for mitochondrial genetic disorders.
    • The development of αDdCBEs represents a significant advancement in mitochondrial gene editing tools.