Related Experiment Video
Updated: Jun 25, 2025
07:11
Constructing Cyclic Peptides Using an On-Tether Sulfonium Center
Published on: September 28, 2022
2.7K
Kinetic Analysis of Lanthipeptide Cyclization by Substrate-Tolerant ProcM
Emily K Desormeaux1, Wilfred A van der Donk1,2
1Department of Chemistry, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue, Urbana, Illinois 61801, USA.
Biorxiv : the Preprint Server for Biology
|May 27, 2024
Summary
This study shows that modification rates can predict lanthipeptide ring patterns. However, these rates do not reliably predict the order of modifications for ProcM synthetase variants.
Area of Science:
- Biochemistry
- Molecular Biology
- Natural Product Synthesis
Background:
- Lanthipeptides are ribosomally synthesized peptides with thioether crosslinks.
- Class II lanthipeptide synthetases, like ProcM, perform complex post-translational modifications.
- ProcM exhibits broad substrate tolerance and high accuracy in forming (methyl)lanthionine rings.
Conclusions:
- The study confirms that modification rates are key determinants of the final lanthipeptide ring pattern.
- However, these rates are not consistently predictive of the modification order in ProcM variants.
- This highlights the complex interplay between substrate sequence, enzyme kinetics, and product formation in lanthipeptide biosynthesis.
Related Concept Videos
00:53Olefin Metathesis Polymerization: Acyclic Diene Metathesis (ADMET)
1.9K
Acyclic diene metathesis polymerization or ADMET polymerization involves cross-metathesis of terminal dienes, such as 1,8-nonadiene, to give linear unsaturated polymer and ethylene. As ADMET is a reversible process, the formed ethylene gas must be removed from the reaction mixture to complete the polymerization process.
Similar to cross-metathesis, ADMET also involves the formation of metallacyclobutane intermediate by [2+2] cycloaddition of one of the double bonds of a terminal diene with...
Similar to cross-metathesis, ADMET also involves the formation of metallacyclobutane intermediate by [2+2] cycloaddition of one of the double bonds of a terminal diene with...
1.9K
00:57Cationic Chain-Growth Polymerization: Mechanism
2.3K
The cationic polymerization mechanism consists of three steps: initiation, propagation, and termination. In the initiation step of the polymerization process, the π bond of a monomer gets protonated by the Lewis acid catalyst, which is formed from boron trifluoride and water. The protonation of the π bond generates a carbocation stabilized by the electron‐donating group. In the propagation step, the π bond of the second monomer acts as a nucleophile and attacks the...
2.3K
01:28ATP and Macromolecule Synthesis
5.6K
Biological macromolecules are organic compounds, predominantly composed of carbon atoms. The carbon atoms are covalently bonded with hydrogen, oxygen, nitrogen, and other minor elements. There are four major biological macromolecule classes: carbohydrates, lipids, proteins, and nucleic acids.
Most macromolecules are composed of single subunits, or building blocks, called monomers. The monomers combine with each other using covalent bonds to form larger molecules known as polymers.
Conversion of...
Most macromolecules are composed of single subunits, or building blocks, called monomers. The monomers combine with each other using covalent bonds to form larger molecules known as polymers.
Conversion of...
5.6K
01:04Anionic Chain-Growth Polymerization: Mechanism
2.0K
The mechanism for anionic chain-growth polymerization involves initiation, propagation, and termination steps. In the initiation step, a nucleophilic anion, such as butyl lithium, initiates the polymerization process by attacking the π bond of the vinylic monomer. As a result, a carbanion, stabilized by the electron‐withdrawing group, is generated. The resulting carbanion acts as a Michael donor in the propagation step and attacks the second vinylic monomer, which acts as a Michael...
2.0K