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Updated: Jun 25, 2025

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

Published on: September 17, 2017

Investigating and Quantifying Molecular Complexity Using Assembly Theory and Spectroscopy.

Michael Jirasek¹, Abhishek Sharma¹, Jessica R Bame¹

¹School of Chemistry, The University of Glasgow, University Avenue, Glasgow G12 8QQ, U.K.

ACS Central Science

|May 27, 2024

Summary

This summary is machine-generated.

Researchers developed a method to experimentally measure molecular complexity using spectroscopy. This approach quantifies molecular assembly, offering new insights into molecular evolution and drug discovery.

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Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies

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Published on: November 28, 2017

Area of Science:

Chemistry
Biophysics
Computer Science

Background:

Current methods for assessing molecular complexity rely on theoretical computer science principles, lacking experimental validation.
Understanding molecular complexity is crucial for fields like drug discovery, origin of life studies, and artificial life research.
Assembly theory provides a framework to quantify molecular complexity via the molecular assembly index (MA), representing the shortest construction path.

Purpose of the Study:

To introduce a novel, rapid method for inferring the molecular assembly index (MA) of molecules directly from spectroscopic data.
To establish an experimentally measurable approach for quantifying molecular complexity.

Main Methods:

Utilized three independent spectroscopic techniques: nuclear magnetic resonance (NMR), tandem mass spectrometry (MS/MS), and infrared spectroscopy (IR).
Analyzed specific spectral features: number of IR absorbances, carbon resonances in NMR, and molecular fragments in MS/MS.
Developed a method to reliably estimate the MA of unknown molecules based on these spectroscopic analyses.

Main Results:

Demonstrated that the molecular assembly index (MA) can be experimentally measured using NMR, MS/MS, and IR spectroscopy.
Showed that analyzing specific spectral features allows for reliable estimation of a molecule's MA.
Established the first experimentally quantifiable method for determining molecular assembly.

Conclusions:

This work presents the first experimental quantification of molecular assembly, moving beyond theoretical measures.
The developed spectroscopic approach enables the study of complex molecule evolution and serves as a marker for evolutionary processes.
This opens new avenues for investigating molecular complexity in various scientific domains, including synthetic biology and astrobiology.