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Related Experiment Video

Updated: Jun 25, 2025

Longitudinal Morphological and Physiological Monitoring of Three-dimensional Tumor Spheroids Using Optical Coherence Tomography

Longitudinal Morphological and Physiological Monitoring of Three-dimensional Tumor Spheroids Using Optical Coherence Tomography

Published on: February 9, 2019

Fully Automated OCT-based Tissue Screening System.

Shaohua Pi^1,2, Razieh Ganjee¹, Lingyun Wang^1,2

¹Department of Ophthalmology, University of Pittsburgh, Pittsburgh, PA 15213, USA.

|May 27, 2024

Summary

This summary is machine-generated.

A new optical coherence tomography (OCT) system enables automated, high-throughput screening of ex vivo tissue cultures. This advanced imaging technology accelerates drug discovery and research by providing rapid, reliable tissue analysis.

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Area of Science:

Biomedical Engineering
Ophthalmology
Drug Discovery

Background:

High-throughput screening is crucial for drug discovery and understanding disease mechanisms.
Current methods for analyzing ex vivo tissue cultures can be time-consuming and labor-intensive.
There is a need for non-invasive, high-resolution imaging techniques for rapid tissue assessment.

Approach:

Developed a novel optical coherence tomography (OCT) imaging system for automated, high-throughput screening of ex vivo tissue cultures.
Integrated a custom motorized platform and tissue detection for successive, automated sample imaging.
Employed transformer-based deep learning for robust segmentation and data analysis.

Key Points:

The system offers non-invasive, high-resolution imaging of tissue architecture and cellular morphology.
Automated imaging and deep learning analysis ensure rapid, consistent, and unbiased data acquisition.
Validated in mouse retinal degeneration models, demonstrating reliable assessment of treatment responses.

Conclusions:

This automated OCT system significantly advances ex vivo tissue screening capabilities.
The technology has the potential to accelerate drug discovery pipelines and biomedical research.
It provides a robust platform for comprehensive, quantitative analysis of tissue responses.