Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Functional Characterization of CsGT3 in the Biosynthesis of (<i>Z</i>)-3-Hexenyl Disaccharides, Anti-Herbivore Metabolites in Tea Plants (<i>Camellia sinensis</i>).

Journal of agricultural and food chemistry·2026
Same author

Rapid neutralization test based on ELISPOT for detecting antibodies against rhinovirus 1B.

Journal of microbiological methods·2026
Same author

DANGER/ITPRIP-mediated iron homeostasis mediates ferroptosis resistance and presents a potential therapeutic target in ccRCC.

Free radical biology & medicine·2026
Same author

Association between extreme temperature exposure and COPD health outcomes in China: study protocol for a systematic review.

Frontiers in medicine·2026
Same author

Construction of a prognostic model for colorectal cancer based on clinical parameters and quantitative body composition using CT imaging.

BMC medical imaging·2026
Same author

A turn-on fluorescent nucleoside enabling sequence-insensitive DNA labeling.

Nucleic acids research·2026

Related Experiment Video

Updated: Jun 25, 2025

Preparation of Exosomes for siRNA Delivery to Cancer Cells
09:59

Preparation of Exosomes for siRNA Delivery to Cancer Cells

Published on: December 5, 2018

25.1K

Large-Area Silicon Nitride Nanosieve for Enhanced Diffusion-Based Exosome Isolation.

Gijung Kim1,2, Mingyu Seo1,2, Jiaxin Xu1

  • 1Department of Biomedical Engineering, Korea University, 466 Hana Science Hall, Seoul, 02841, Republic of Korea.

Small Methods
|May 27, 2024
PubMed
Summary

Researchers developed large-area silicon nitride nanosieves for efficient exosome isolation. These thin, high-porosity membranes significantly improve nanoparticle recovery and purity for therapeutic applications.

Keywords:
diffusion‐based filtrationexosome separationnano‐porous membranesilicon‐nitride membrane

More Related Videos

Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles
10:16

Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles

Published on: January 20, 2023

3.0K
Porous Silicon Microparticles for Delivery of siRNA Therapeutics
08:31

Porous Silicon Microparticles for Delivery of siRNA Therapeutics

Published on: January 15, 2015

10.9K

Related Experiment Videos

Last Updated: Jun 25, 2025

Preparation of Exosomes for siRNA Delivery to Cancer Cells
09:59

Preparation of Exosomes for siRNA Delivery to Cancer Cells

Published on: December 5, 2018

25.1K
Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles
10:16

Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles

Published on: January 20, 2023

3.0K
Porous Silicon Microparticles for Delivery of siRNA Therapeutics
08:31

Porous Silicon Microparticles for Delivery of siRNA Therapeutics

Published on: January 15, 2015

10.9K

Area of Science:

  • Materials Science
  • Biotechnology
  • Nanotechnology

Background:

  • Nanoporous membranes are crucial for size-selective nanoparticle separation, particularly for isolating exosomes in drug delivery.
  • Commercial membranes face limitations in pore density and thickness, hindering exosome isolation efficiency and practicality.
  • Previous attempts at thin membrane fabrication were restricted by limited surface area.

Purpose of the Study:

  • To present large-area silicon nitride nanosieves for enhanced diffusion-based exosome isolation.
  • To overcome fabrication challenges for scalable, high-performance nanoporous membranes.
  • To improve exosome recovery rates and purity for therapeutic applications.

Main Methods:

  • Fabrication of large-area silicon nitride nanosieves up to 4-inch wafers.
  • Utilizing a 200 nm porous sieve (38.2% porosity) for exosome separation.
  • Employing a 50 nm sieve (10.7% porosity) for soluble protein removal.
  • Development of 300 nm thick nanosieves, significantly thinner than conventional membranes.

Main Results:

  • Achieved a 90% recovery rate of intact exosomes from human serum.
  • Obtained a purity ratio of 3 × 10^7 particles/µg protein, 4.6 times higher than ultracentrifugation.
  • Demonstrated increased nanoparticle permeability due to the thin nanosieve design.
  • Showcased high throughput capability up to 15 mL with scalable nanosieve size.

Conclusions:

  • The developed silicon nitride nanosieves offer superior performance compared to conventional membranes.
  • This technology enables efficient, large-scale exosome isolation for therapeutic purposes.
  • The nanosieves represent a significant advancement in exosome purification and production.