The Acute Inflammatory Potential of Particles From the Echinococcus granulosus Laminated Layer Is Moderated by Its...

Area of Science:

• Parasitology
• Immunology
• Biochemistry

Background:

• Cystic echinococcosis is caused by Echinococcus granulosus larvae, protected by an acellular laminated layer (LL).
• LL particles shed by the parasite may induce inflammation, but the roles of its components are unclear.
• Key LL components include glycosylated mucins and calcium inositol hexakisphosphate (InsP6).

Purpose of the Study:

• To investigate the in vivo inflammatory potential of LL particles.
• To determine the specific roles of LL mucin glycans and calcium InsP6 in LL-induced inflammation.

Main Methods:

• Intraperitoneal injection of LL particles into mice.
• Analysis of inflammatory cell infiltration (eosinophils, neutrophils, monocytes/macrophages).
• Comparison of inflammation induced by native LL particles, calcium InsP6-depleted LL particles, and carbohydrate-oxidised LL particles.

Main Results:

• LL particles induced acute inflammation, characterized by eosinophil, neutrophil, and monocyte/macrophage infiltration and loss of resident macrophages.
• Absence of calcium InsP6 significantly enhanced inflammatory cell recruitment.
• Oxidation of mucin carbohydrates reduced neutrophil recruitment but did not abolish particle-induced cell influx.

Conclusions:

• LL particles from Echinococcus granulosus induce acute inflammatory responses.
• Calcium InsP6 component of LL particles attenuates LL-induced inflammation.
• LL mucin glycans contribute to inflammatory cell recruitment, potentially via receptor-independent mechanisms.