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A D-π-A-type ratiometric fluorescent probe to detect polarity changes and inhibition effect during ferroptosis

  • 0School of Chemistry and Chemical Engineering, University of Jinan, Jinan, Shandong 250022, China. ifp_dongbl@ujn.edu.cn.
Analytical Methods : Advancing Methods and Applications +

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May 28, 2024

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May 28, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

Researchers developed a ratiometric probe (Po-P) to track cell polarity changes during ferroptosis, a type of cell death. This tool can visualize ferroptosis and assess potential inhibitors like dihydrolipoic acid.

Area Of Science

  • Biochemistry
  • Cell Biology
  • Chemical Biology

Background

  • Ferroptosis is a unique form of cell death dependent on iron(II).
  • Understanding ferroptosis requires monitoring cellular polarity shifts during this process.
  • Existing methods may not adequately visualize these dynamic changes.

Purpose Of The Study

  • To develop a novel ratiometric probe (Po-P) for visualizing cellular polarity variations.
  • To monitor the inhibition effects during ferroptosis in living cells.
  • To investigate the potential of Po-P in drug discovery for ferroptosis-related disorders.

Main Methods

  • Design and synthesis of a D-π-A type polarity-responsive fluorophore for the Po-P probe.
  • Theoretical calculations to elucidate the intramolecular charge transfer (ICT) mechanism.
  • Live-cell imaging experiments to assess Po-P's performance in monitoring ferroptosis and inhibition.

Main Results

  • The Po-P probe effectively visualized polarity fluctuations during erastin-induced ferroptosis.
  • Po-P demonstrated a capacity for monitoring the inhibition of ferroptosis.
  • Dihydrolipoic acid (DHLA) and vitamin E (VE) were shown to potentially inhibit ferroptosis-induced polarity changes.

Conclusions

  • The Po-P probe is a valuable tool for real-time monitoring of ferroptosis and its inhibition.
  • This probe can aid in the discovery of new therapeutic agents for ferroptosis-associated diseases.
  • Further research using Po-P may deepen the understanding of ferroptosis mechanisms.