Related Experiment Video
Updated: Jun 25, 2025
07:11
A Quantitative Detection Method for MicroRNAs in the Kidney of an Ischemic Kidney Injury Mouse Model
Published on: September 11, 2020
1.4K
A Systematic Review and Meta-Analysis of microRNA Profiling Studies in Chronic Kidney Diseases
Gantsetseg Garmaa1,2,3, Stefania Bunduc2,4,5,6, Tamás Kói2,7
1Institute of Translational Medicine, Semmelweis University, Nagyvárad tér 4, 1089 Budapest, Hungary.
Non-Coding RNA
|May 28, 2024
Summary
This study identified novel microRNA (miRNA) biomarkers for chronic kidney disease (CKD). Key findings highlight specific miRNAs and pathways involved in CKD progression, offering potential therapeutic targets.
Area of Science:
- Nephrology
- Genomics
- Biomarker Discovery
Background:
- Chronic kidney disease (CKD) poses a growing global health challenge.
- MicroRNAs (miRNAs) show promise as diagnostic markers for CKD, but existing research presents inconsistencies.
- Hypothesis-free profiling is needed to identify reliable miRNA biomarkers and therapeutic targets in CKD.
Purpose of the Study:
- To identify novel miRNA biomarkers and potential therapeutic targets for CKD.
- To analyze hypothesis-free miRNA profiling studies across human and murine CKD models.
- To elucidate key molecular pathways implicated in CKD pathogenesis.
Main Methods:
- Conducted comprehensive literature searches across five databases.
- Performed subgroup analyses based on kidney disease type, sample source, disease stage, and species.
- Utilized Robust Rank Aggregation (RRA) and vote-counting for analyzing 38 human and 12 murine studies.
- Conducted gene set enrichment analyses using DIANA-miRPath v4.0 and MIENTURNET.
Main Results:
- Identified 145 dysregulated miRNAs in human CKD and 32 in murine models via vote-counting.
- miR-26a-5p was significantly reduced in Lupus nephritis (LN) kidney tissue; miR-107 decreased in LN blood samples (RRA).
- Enriched pathways across species included epithelial-mesenchymal transition, Notch, mTOR signaling, apoptosis, G2/M checkpoint, and hypoxia.
Conclusions:
- Novel miRNA signatures and target genes associated with various kidney diseases were identified.
- Specific miRNAs like miR-26a-5p and miR-107 show potential as biomarkers for Lupus nephritis.
- Identified pathways provide insights into CKD mechanisms, warranting validation in large cohorts.
More Related Videos
Related Concept Videos
01:17Factors Affecting Renal Clearance: Renal Impairment
86
Renal dysfunction significantly impairs the renal clearance of drugs, leading to potential complications in drug therapy. Renal failure, which can be caused by various factors, poses a significant challenge in the elimination of drugs from the body.
One condition associated with renal failure is uremia. Uremia is characterized by impaired glomerular filtration and fluid accumulation in the body. This condition hinders the renal clearance of drugs, resulting in drug accumulation and potential...
One condition associated with renal failure is uremia. Uremia is characterized by impaired glomerular filtration and fluid accumulation in the body. This condition hinders the renal clearance of drugs, resulting in drug accumulation and potential...
86
01:22MicroRNAs
3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K