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Human and murine interleukin 1 possess sequence and structural similarities
P E Auron1, L J Rosenwasser, K Matsushima
1Harvard-M.I.T. Division of Health Sciences and Technology, Cambridge, MA 02139.
The Journal of Molecular and Cellular Immunology : JMCI
|January 1, 1985
Summary
Comparing human and murine interleukin-1 (IL-1) precursor sequences reveals conserved regions critical for biological activity. This analysis identifies key areas essential for IL-1 function, aiding in understanding its molecular mechanisms.
Area of Science:
- Immunology
- Molecular Biology
- Biochemistry
Background:
- Human and murine interleukin-1 (IL-1) precursor sequences have been molecularly cloned and analyzed.
- Comparing these sequences can identify conserved regions vital for biological activity.
Purpose of the Study:
- To confirm the relationship between human and murine IL-1 molecules.
- To identify specific regions essential for IL-1 biological activity.
Main Methods:
- Comparative analysis of amino acid sequences from human and murine IL-1 precursor.
- Amino terminal sequence analysis of biologically active IL-1 isolated from human monocytes.
Main Results:
- The study confirmed a relationship between human and murine IL-1 molecules.
- Specific conserved regions were suggested to be essential for IL-1 activity.
- Amino terminal sequence analysis of human IL-1 aligned with human cDNA predictions.
- The processed amino terminus of human IL-1 was located similarly to the murine sequence.
Conclusions:
- Comparative sequence analysis is valuable for identifying functionally important regions in IL-1.
- The findings support the hypothesis that specific molecular regions dictate IL-1's biological activity.