Protocol for changing gene expression in 3T3-L1 (pre)adipocytes using siRNA-mediated knockdown
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Updated: Jun 25, 2025
An Adipocyte Cell Culture Model to Study the Impact of Protein and Micro-RNA Modulation on Adipocyte Function
Published on: May 4, 2021
Martina Strnadová1, Doreen Thor1, Isabell Kaczmarek1
1Rudolf Schönheimer Institute of Biochemistry, Medical Faculty, Leipzig University, Leipzig, 04103 Saxony, Germany.
Abstract:
3T3-L1 is a model cell line which can be differentiated from preadipocytes into mature adipocytes. Here, we present a protocol for changing gene expression in 3T3-L1 (pre)adipocytes using small interfering RNA (siRNA)-mediated knockdown. We describe steps to perform the knockdown of a certain gene prior to differentiation (day 4) to analyze the impact on adipogenesis. We then detail procedures for knockdown on day 8 of differentiation to study the role of a certain gene in mature adipocyte function. For complete details on the use and execution of this protocol, please refer to Kaczmarek et al.1.
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