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Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...

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Updated: May 4, 2026

High-throughput Protein Expression Generator Using a Microfluidic Platform

High-throughput Protein Expression Generator Using a Microfluidic Platform

Published on: August 23, 2012

mRNA Display Pipeline for Protein Biosensor Construction.

Zhenling Cui^1,2,3, Cagla Ergun Ayva^2,3, Yi Jin Liew⁴

¹ARC Centre of Excellence in Synthetic Biology, Brisbane, Queensland 4001, Australia.

|May 29, 2024

Summary

This summary is machine-generated.

Developing modular protein biosensors is streamlined by a new workflow. This method rapidly generates specific binders, improving biosensor assembly and prototyping for liver toxicity markers.

Keywords:

DARPins Monobodies mRNA display protein biosensor synthetic protein binding domain

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Area of Science:

Biotechnology
Molecular Biology
Protein Engineering

Background:

Protein biosensor development is often inefficient, relying on trial-and-error methods.
Modular biosensor designs offer a promising avenue for creating adaptable biosensors.
The integration of specific binding domains into biosensor architectures is crucial for target recognition.

Purpose of the Study:

To establish and validate a high-throughput workflow for generating specific binders for protein biosensors.
To develop novel biosensors for detecting liver toxicity markers using the established workflow.
To investigate the relationship between binder affinity and biosensor performance.

Main Methods:

Utilized mRNA display and ribosome display for selection of hyper-stable monobody domains and DARPins, respectively.
Integrated selected binders into a two-component allosteric biosensor architecture featuring a calmodulin-reporter chimera.
Tested the workflow by developing biosensors for liver toxicity markers: aspartate aminotransferase (cytosolic and mitochondrial) and alanine aminotransferase 1.

Main Results:

The workflow consistently generated over 10^3 unique binders per target within one week.
Binder affinity for the target did not directly correlate with biosensor performance.
Interactions between binding domains and the reporter module significantly influenced biosensor activity and dynamic range.

Conclusions:

The developed workflow offers an efficient method for generating specific binders for protein biosensor construction.
Multiplexed biosensor assembly and prototyping are critical steps for optimizing biosensor function post-binding domain selection.
This approach holds promise for accelerating the development of custom protein biosensors for various applications, including diagnostics.