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True renin in human pituitary tissue.

K Mizuno, M Ojima, M Gotoh

    Journal of Neurochemistry
    |February 1, 1985
    PubMed
    Summary

    True renin, an enzyme typically found in the kidneys, was detected in human pituitary tissues. This pituitary renin shares biochemical properties with kidney renin, suggesting its presence in the pituitary gland.

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    Area of Science:

    • Endocrinology
    • Biochemistry
    • Molecular Biology

    Background:

    • Renin is a key enzyme in the renin-angiotensin system, primarily known for its role in blood pressure regulation and produced mainly by the kidneys.
    • The presence and function of renin in the human pituitary gland are not well-established.
    • Investigating non-renal renin activity is crucial for understanding broader physiological roles.

    Purpose of the Study:

    • To determine if renin activity is present in human pituitary tissue.
    • To characterize the biochemical properties of pituitary renin.
    • To ascertain if pituitary renin is biochemically similar to renal renin.

    Main Methods:

    • Measurement of enzymatic activity in human pituitary tissue extracts.
    • Inhibition assays using antibodies specific to human renal renin.
    • Biochemical characterization including molecular weight determination and optimum pH analysis.
    • Assessment of inactive renin activation by trypsin.

    Main Results:

    • Detectable levels of renin activity were found in human pituitary tissue.
    • The observed activity was specifically inhibited by an antibody against human renal renin, ruling out non-specific protease activity.
    • Pituitary renin exhibited biochemical similarities to kidney renin, including molecular weight, optimal pH, and the presence of a trypsin-activatable inactive form.

    Conclusions:

    • The findings strongly suggest the existence of true renin within human pituitary tissue.
    • Pituitary renin shares significant biochemical characteristics with renal renin.
    • This discovery opens avenues for exploring the potential role of pituitary renin in local or systemic physiological processes.

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