Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

4.5K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.5K
Negative Regulator Molecules01:23

Negative Regulator Molecules

35.3K
Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
35.3K
DNA Damage can Stall the Cell Cycle02:37

DNA Damage can Stall the Cell Cycle

9.1K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
9.1K
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

6.3K
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
6.3K
Epigenetic Regulation01:37

Epigenetic Regulation

3.0K
Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
3.0K
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

4.7K
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
4.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Atlantoaxial subluxation treated with exercise therapy combined with occipito-maxillary band traction: a case report.

Journal of medical case reports·2026
Same author

Psychological-Symptom Mismatch Phenotypes and Their Clinical Implications in Patients with Chronic Obstructive Pulmonary Disease.

International journal of chronic obstructive pulmonary disease·2026
Same author

Is Messier Better? Mechanistic Insights toward Ultracryogenic Metal-Ion Batteries.

Journal of the American Chemical Society·2026
Same author

Beyond Identifier Matching: An Empirical Characterization of Failure Modes in Biomedical Knowledge Graph Integration.

medRxiv : the preprint server for health sciences·2026
Same author

Development and validation of a plasma miRNA-CEA biomarker panel for early detection of lung cancer.

Cancer treatment and research communications·2026
Same author

Corona Chain-Controlled Transition from Ostwald Ripening-Grown Hexagonal Platelets to Screw-Dislocation Spirals in Liquid-Crystalline Polypeptoids.

Nano letters·2026

Related Experiment Video

Updated: Jun 25, 2025

Yeast As a Chassis for Developing Functional Assays to Study Human P53
14:57

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

9.5K

p53 exerts anticancer effects by regulating enhancer formation and activity.

Shuhan Chen1,2, Xuchun Wang1,2, Nan Yang1

  • 1Key Laboratory of Human Functional Genomics of Jiangsu Province, School of Basic Medical Sciences, Nanjing Medical University, Nanjing, Jiangsu 211166, China.

Journal of Biomedical Research
|May 29, 2024
PubMed
Summary
This summary is machine-generated.

The p53 tumor suppressor regulates enhancers critical for combating lung cancer. Loss of p53 disrupts these enhancers, impacting KLF4 expression and promoting cell growth, suggesting a novel therapeutic strategy.

Keywords:
enhancermalignant transformationp53tumor

More Related Videos

Purification of Ubiquitinated p53 Proteins from Mammalian Cells
10:55

Purification of Ubiquitinated p53 Proteins from Mammalian Cells

Published on: March 21, 2022

2.1K
Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method
09:32

Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method

Published on: September 10, 2017

11.0K

Related Experiment Videos

Last Updated: Jun 25, 2025

Yeast As a Chassis for Developing Functional Assays to Study Human P53
14:57

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

9.5K
Purification of Ubiquitinated p53 Proteins from Mammalian Cells
10:55

Purification of Ubiquitinated p53 Proteins from Mammalian Cells

Published on: March 21, 2022

2.1K
Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method
09:32

Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method

Published on: September 10, 2017

11.0K

Area of Science:

  • Molecular Biology
  • Genomics
  • Oncology

Background:

  • The p53 tumor suppressor is vital in lung cancer, regulating genes to prevent malignancy.
  • p53 binding to enhancers is known, but its role in shaping the enhancer landscape is unclear.

Purpose of the Study:

  • To investigate how p53 influences enhancer activity and the broader enhancer landscape in lung epithelial cells.
  • To elucidate the functional consequences of p53-mediated enhancer regulation in lung cancer development.

Main Methods:

  • Utilized functional genomics in TP53 knockout (KO) human bronchial epithelial cells (BEAS-2B).
  • Assessed enhancer activity at p53 binding sites genome-wide.
  • Employed a cell transformation model using nitrosamine exposure.

Main Results:

  • Deletion of p53 led to the disappearance of 943 active enhancers and 370 super-enhancers (SEs).
  • A p53-dependent SE (KLF4-SE) was identified, regulating KLF4 expression.
  • Loss of p53 decreased KLF4-SE activity and KLF4 levels, increasing cell transformation and colony formation; KLF4 overexpression partially reversed this.

Conclusions:

  • p53 modulates hundreds of enhancer elements, indicating its role in shaping the enhancer landscape.
  • p53's regulation of enhancers, particularly KLF4-SE, is integral to its tumor suppressor function in lung cancer.
  • Findings offer insights into p53's mechanism in lung oncogenesis and suggest new therapeutic targets.