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Smartphone-based optical sectioning (SOS) microscopy with a telecentric design for fluorescence imaging.

Ziao Jiao1,2, Mingliang Pan1, Khadija Yousaf3

  • 1Department of Biomedical Engineering, University of Strathclyde, Glasgow, Scotland, UK.

Journal of Microscopy
|May 29, 2024
PubMed
Summary
This summary is machine-generated.

We developed a low-cost smartphone optical sectioning microscope using the HiLo technique. This innovative system provides high-contrast imaging and optical sectioning, making advanced microscopy accessible for biomedical research.

Keywords:
HiLo microscopyoptical sectioning microscopysmartphone microscopy

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Area of Science:

  • Biomedical Optics
  • Microscopy Technology

Background:

  • Traditional HiLo microscopy systems are expensive and complex.
  • There is a need for cost-effective and portable microscopy solutions for diverse research settings.

Purpose of the Study:

  • To develop a smartphone-based optical sectioning microscope (SOS) utilizing the HiLo technique.
  • To demonstrate the feasibility of using a smartphone as a core component for illumination and detection.
  • To achieve high-contrast imaging and optical sectioning capabilities at a significantly reduced cost.

Main Methods:

  • Integration of a smartphone with off-the-shelf optical and mechanical components using 3D-printed adapters.
  • Utilizing the smartphone's LED as a light source coupled to a digital mirror device (DMD).
  • Employing an electrically tunable lens (ETL) for low-cost axial scanning within a telecentric 4f configuration.

Main Results:

  • The smartphone-based optical sectioning microscope (SOS) achieved a 571.5 µm telecentric scanning range and 11.7 µm axial resolution.
  • Successful high-contrast imaging of fluorescent polystyrene beads with different wavelengths.
  • Demonstrated optical sectioning of multilayer fluorescent polystyrene beads.

Conclusions:

  • The proposed smartphone-based HiLo optical sectioning microscopy is the first of its kind, costing significantly less than traditional systems.
  • This technology offers a powerful and accessible tool for biomedical research, particularly in resource-limited environments.
  • The SOS system provides a viable alternative for advanced imaging applications requiring optical sectioning.