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Research Domains Agricultural, Veterinary and Food Sciences Agricultural Biotechnology Agricultural biotechnology diagnostics (incl. biosensors)
Unveiling the traits of antibiotic resistance and virulence in Escherichia coli obtained from poultry waste

Unveiling the traits of antibiotic resistance and virulence in Escherichia coli obtained from poultry waste

Sahil Yoginath Bhambure ¹, Lakiesha Inacia Coelho E Costa ¹, Ashwitha M Gatty ¹, Kavitha Guladahalli Manjunatha ¹, Rajeshwari Vittal ¹, Akhila Dharnappa Sannejal ²

Sahil Yoginath Bhambure ¹, Lakiesha Inacia Coelho E Costa ¹, Ashwitha M Gatty ¹

¹Nitte (Deemed to be University), Nitte University Centre for Science Education and Research (NUCSER), Paneer campus, Deralakatte, Mangalore, 575018, India.
²Nitte (Deemed to be University), Nitte University Centre for Science Education and Research (NUCSER), Paneer campus, Deralakatte, Mangalore, 575018, India. akhila@nitte.edu.in.

⁰Nitte (Deemed to be University), Nitte University Centre for Science Education and Research (NUCSER), Paneer campus, Deralakatte, Mangalore, 575018, India.

Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology] +

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May 29, 2024

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View abstract on PubMed

Summary

This summary is machine-generated.

Avian pathogenic Escherichia coli (APEC) in poultry waste harbors antibiotic resistance and virulence genes, posing environmental pollution risks. Molecular screening revealed APEC strains resistant to common antibiotics, highlighting their ecological impact.

Area Of Science

Veterinary Microbiology
Environmental Science
Molecular Biology

Background

Antibiotic resistance and virulence factors in avian pathogenic Escherichia coli (APEC) are significant environmental concerns.
Extensive antibiotic use in poultry farming drives the emergence of resistant APEC strains.

Purpose Of The Study

To molecularly screen APEC from poultry waste for antibiotic resistance and virulence attributes.
To assess the environmental impact of APEC, focusing on its role as a reservoir for resistance and virulence genes.

Main Methods

Analysis of 50 poultry waste samples (litter, feces, wastewater, anatomical waste).
Molecular characterization of 35 Escherichia coli isolates for virulence genes.
Identification of antibiotic resistance patterns in APEC strains.

Main Results

27 of 35 E. coli isolates were identified as APEC, each possessing at least four virulence genes.
Commonly detected virulence genes include fimH, ompA, ybjX, waaL, cvaC, hlyF, iss, ompT, and iroN.
Eleven APEC strains showed resistance to tetracycline, ampicillin, sulphonamides, and fluoroquinolones.

Conclusions

APEC in poultry waste acts as a significant source of environmental pollution and a reservoir for virulence and resistance genes.
Understanding APEC dynamics is crucial for mitigating environmental pollution and safeguarding public health via the food chain.
Effective strategies are needed to address the ecological impact of APEC and protect ecosystems and human populations.

Keywords:

Antibiotic resistance Avian pathogenic Escherichia coli Food chain, virulence Poultry wastes

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Antibiotic Selection

Antibiotic Selection

Overview

Researchers use antibiotic resistance genes to identify bacteria that possess a plasmid containing their gene of interest. Antibiotic resistance naturally occurs when a spontaneous DNA mutation creates changes in bacterial genes that eliminate antibiotic activity. Bacteria can share these new resistance genes with their offspring and other bacteria. The overuse and misuse of antibiotics have created a public health crisis, as resistant and multi-resistant bacteria continue to develop.

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