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Aaptamine-rich Fraction from the Indonesian Marine Sponge, Aaptos suberitoides, Exhibits a Cytotoxic Effect on DLD-1

Mutia Hardhiyuna1, Ucu Yanu Arbi2, Zuraida Zuraida1

  • 1Research Center for Vaccine and Drugs, Research Organization for Health, National Research and Innovation Agency (BRIN), Jl. Raya Bogor Km.46, Cibinong, 16911, Indonesia.

Asian Pacific Journal of Cancer Prevention : APJCP
|May 29, 2024
PubMed
Abstract

Insights

The ethyl acetate extract of the Aaptos suberitoides sponge shows promising anticancer properties. It effectively targets colorectal cancer cells (DLD-1) with greater toxicity than normal murine fibroblast cells (NIH-3T3).

Area of Science:

  • Marine Natural Products Chemistry
  • Cancer Biology
  • Drug Discovery

Background:

  • Marine sponges are a rich source of bioactive compounds.
  • Aaptos suberitoides is a marine sponge with potential pharmacological applications.
  • Colorectal cancer remains a significant global health challenge.

Purpose of the Study:

  • To evaluate the cytotoxic effects of the ethyl acetate extract of Aaptos suberitoides.
  • To assess the extract's efficacy against colorectal cancer cells (DLD-1) and normal murine fibroblast cells (NIH-3T3).

Main Methods:

  • Aaptos suberitoides was collected and extracted using ethyl acetate.
  • Extracts were analyzed using Thin Layer Chromatography (TLC) and Liquid Chromatography-Mass Spectrometry/Mass Spectrometry (LCMS/MS).
  • Cytotoxicity was determined using the MTT assay on DLD-1 and NIH-3T3 cell lines.

Main Results:

  • LCMS/MS analysis identified aaptamine as a major compound in the extract.
  • The ethyl acetate extract exhibited significant cytotoxicity against DLD-1 cells (IC50 = 9.597 µg/mL).
  • The extract was less toxic to NIH-3T3 cells (IC50 = 12.23 µg/mL), indicating selective toxicity.

Conclusions:

  • The ethyl acetate extract of Aaptos suberitoides demonstrates selective cytotoxicity towards colorectal cancer cells.
  • Aaptamine is a key compound contributing to the observed anticancer activity.
  • This extract shows potential for development as a colorectal anticancer therapeutic agent.
Keywords:
Cytotoxicaaptaminecolorectal cancermarine sponge

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