Abnormal expression of serum miR-4746-5p in liver cancer patients after interventional chemotherapy and its possible...

Area of Science:

• Oncology
• Molecular Biology
• Biochemistry

Background:

• Interventional chemotherapy is a standard treatment for liver cancer.
• Understanding molecular mechanisms influencing treatment response is crucial.

Purpose of the Study:

• To investigate serum miR-4746-5p expression and its molecular mechanism in liver cancer patients undergoing interventional chemotherapy.
• To evaluate miR-4746-5p as a potential biomarker for diagnosis and treatment response.

Main Methods:

• Serum samples analyzed for miR-4746-5p and CDKN1C using real-time quantitative PCR.
• Diagnostic value assessed via Receiver Operating Characteristic (ROC) curves.
• Molecular interactions confirmed using luciferase reporter gene assays and in vitro cellular assays (CCK-8, Transwell, chemoresistance).

Main Results:

• Serum miR-4746-5p was upregulated in liver cancer patients, decreasing post-chemotherapy.
• CDKN1C expression showed an inverse correlation with miR-4746-5p levels.
• miR-4746-5p negatively regulated CDKN1C, impacting cell proliferation, migration, and chemoresistance.

Conclusions:

• Serum miR-4746-5p serves as a potential diagnostic biomarker for liver cancer.
• miR-4746-5p's modulation of CDKN1C suggests a role in liver cancer progression and chemotherapy response.
• miR-4746-5p may represent a therapeutic target for improving liver cancer treatment outcomes.