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Updated: Jun 25, 2025

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
Published on: May 3, 2018
Characterization and functional analysis of chicken CDK protein
Zhixuan Xiong1, Jingjing Cao2, Ke Wang3
1Department of Veterinary Preventive Medicine, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, 330045, China; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.
Chicken cell cycle-dependent kinases (CDKs), especially CDK6, suppress the innate immune response. These CDKs facilitate infectious bursal disease virus (IBDV) replication by inhibiting interferon-beta (IFN-β) production.
Area of Science:
- Immunology
- Virology
- Molecular Biology
Background:
- Cell cycle-dependent kinases (CDKs) regulate cell cycle progression and are known to influence mammalian innate immunity and viral replication.
- The specific roles of CDKs in chicken innate immune responses and viral pathogenesis are not well understood.
Purpose of the Study:
- To investigate the function of chicken CDKs in innate immunity and infectious bursal disease virus (IBDV) replication.
- To determine the role of CDK6 in regulating interferon-beta (IFN-β) production and IBDV replication in chickens.
Main Methods:
- Cloning of chicken CDK6-CDK10 genes.
- Expression analysis of CDK6 in various chicken tissues and DF-1 cells.
- Assessment of chicken CDK effects on IFN-β signaling pathways (MAVS, STING, MDA5).
- Analysis of CDK recruitment during IBDV infection and co-localization with viral protein VP1.
- Overexpression and knockdown studies of CDK6 in chickens to evaluate IBDV replication and IFN-β production.
Main Results:
- CDK6 is broadly expressed in chicken tissues and DF-1 cells.
- Multiple chicken CDKs negatively regulate IFN-β signaling.
- Chicken CDKs, except CDK10, co-localize with IBDV protein VP1 during infection.
- CDK6 overexpression enhances IBDV replication, while CDK6 knockdown increases IFN-β production and reduces IBDV replication.
Conclusions:
- Chicken CDKs, particularly CDK6, act as negative regulators of IFN-β production.
- CDK6 plays a facilitative role in IBDV replication in chickens.
- Targeting CDK6 presents a potential strategy for controlling IBDV infections.
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