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Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
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Published on: May 3, 2018

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Characterization and functional analysis of chicken CDK protein

Zhixuan Xiong1, Jingjing Cao2, Ke Wang3

  • 1Department of Veterinary Preventive Medicine, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, 330045, China; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, 430070, China.

Poultry Science
|May 29, 2024
PubMed

Insights

Chicken cell cycle-dependent kinases (CDKs), especially CDK6, suppress the innate immune response. These CDKs facilitate infectious bursal disease virus (IBDV) replication by inhibiting interferon-beta (IFN-β) production.

Area of Science:

  • Immunology
  • Virology
  • Molecular Biology

Background:

  • Cell cycle-dependent kinases (CDKs) regulate cell cycle progression and are known to influence mammalian innate immunity and viral replication.
  • The specific roles of CDKs in chicken innate immune responses and viral pathogenesis are not well understood.

Purpose of the Study:

  • To investigate the function of chicken CDKs in innate immunity and infectious bursal disease virus (IBDV) replication.
  • To determine the role of CDK6 in regulating interferon-beta (IFN-β) production and IBDV replication in chickens.

Main Methods:

  • Cloning of chicken CDK6-CDK10 genes.
  • Expression analysis of CDK6 in various chicken tissues and DF-1 cells.
  • Assessment of chicken CDK effects on IFN-β signaling pathways (MAVS, STING, MDA5).
  • Analysis of CDK recruitment during IBDV infection and co-localization with viral protein VP1.
  • Overexpression and knockdown studies of CDK6 in chickens to evaluate IBDV replication and IFN-β production.

Main Results:

  • CDK6 is broadly expressed in chicken tissues and DF-1 cells.
  • Multiple chicken CDKs negatively regulate IFN-β signaling.
  • Chicken CDKs, except CDK10, co-localize with IBDV protein VP1 during infection.
  • CDK6 overexpression enhances IBDV replication, while CDK6 knockdown increases IFN-β production and reduces IBDV replication.

Conclusions:

  • Chicken CDKs, particularly CDK6, act as negative regulators of IFN-β production.
  • CDK6 plays a facilitative role in IBDV replication in chickens.
  • Targeting CDK6 presents a potential strategy for controlling IBDV infections.
Keywords:
CDKIBDVIFN-βchickenreplication

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