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16S rRNA sequencing in chronic dacryocystitis.

Yongxin Zhang1, Beian Liu2, Meina Yang1

  • 1Shenzhen Eye Hospital, Jinan University, Shenzhen Eye Institute, Shenzhen, Guangdong, China.

Clinical & Experimental Optometry
|May 29, 2024
PubMed
Summary

Investigating the microflora of chronic dacryocystitis (CDC) using 16S rRNA sequencing reveals distinct bacterial compositions in healthy and affected eyes. These findings offer insights into CDC pathogenesis and potential antibiotic treatment strategies.

Keywords:
16S rRNA sequencingchronic dacryocystitismicroflora

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Area of Science:

  • Ophthalmology
  • Microbiology
  • Genomics

Background:

  • Chronic dacryocystitis (CDC) pathogenesis is linked to diverse bacterial involvement.
  • Understanding ocular microflora can inform CDC prevention and treatment strategies.
  • 16S rRNA sequencing, a bacterial analysis method, has limited application in CDC studies.

Purpose of the Study:

  • To investigate the bacterial microflora in healthy and affected eyes of CDC patients.
  • To analyze differences in conjunctival sac and lacrimal sac secretions.
  • To explore potential microbial functions using 16S rRNA sequencing and PICRUSt.

Main Methods:

  • A case-control study involving 78 patients with CDC.
  • Samples collected from healthy eyes (Group A), affected conjunctival sacs (Group B), and affected lacrimal sacs (Group C).
  • Bacterial flora analyzed using 16S rRNA sequencing, with data processed by QIIME, R, and LefSE; functional analysis by PICRUSt.

Main Results:

  • 1440 operational taxonomic units (OTUs) identified, with specific OTUs unique to each group.
  • No significant difference in alpha diversity across groups; beta diversity analysis showed compositional differences.
  • Distinct bacterial abundances identified in each group: e.g., Pseudomonadaceae in Group A, Enterobacteriaceae in Groups B and C, and Streptococcus digestiveis in Group C.

Conclusions:

  • Alterations in conjunctival and lacrimal sac microbiota are associated with CDC pathogenesis.
  • Specific bacterial compositions in different ocular sites may guide antibiotic therapy for CDC.
  • This study provides a foundation for understanding the role of microflora in CDC.