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Bone marrow from cadaver donors for transplantation.
Blood
|February 1, 1985
Summary
Bone marrow cells from cadaver donors can be successfully retrieved for transplantation. Depleting T cells from this marrow may enable its use in allogeneic transplants, potentially requiring enhanced immunosuppression.
Area of Science:
- Hematology
- Transplantation immunology
- Cellular therapy
Background:
- Bone marrow transplantation is a critical treatment for various hematologic disorders.
- The scarcity of suitable living donors necessitates exploring alternative sources like cadaveric donors.
- Cadaver marrow presents unique challenges, including potential immunogenicity and cell viability.
Purpose of the Study:
- To assess the feasibility of obtaining viable bone marrow cells from cadaver donors for transplantation.
- To evaluate the effectiveness of T cell depletion in cadaver marrow.
- To determine the yield of hematopoietic stem cells after processing.
Main Methods:
- Marrow cells were isolated from 17 cadaver donors after iliac crest removal.
- T cells were depleted using a monoclonal antibody and human complement.
- Stem cell yields were quantified using colony-forming assays (CFU-C, CFU-E, BFU-E, CFU-GEMM).
Main Results:
- Up to 2 x 10^9 cells were isolated per donor.
- Cadaver marrow showed a lower T cell percentage (10%) compared to living donors.
- Despite cell loss during processing, sufficient stem cells were recovered: CFU-C (84%), CFU-E (39%), BFU-E (81%), CFU-GEMM (48%).
Conclusions:
- T cell-depleted bone marrow from cadaver donors is a potentially viable source for transplantation.
- Further research into improved immunosuppressive strategies is needed to mitigate graft rejection risks due to histocompatibility differences.