Characterization of an endoplasmic reticulum stress-associated lncRNA prognostic signature and the tumor-suppressive role of RP11-295G20.2 knockdown in lung adenocarcinoma
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View abstract on PubMed
Summary
This summary is machine-generated.This study identifies nine endoplasmic reticulum stress-related long non-coding RNAs (lncRNAs) that predict prognosis in lung adenocarcinoma (LUAD). A risk model using these lncRNAs highlights differences in tumor progression and immunity between high- and low-risk LUAD patients.
Area Of Science
- Oncology
- Molecular Biology
- Genomics
Background
- Endoplasmic reticulum stress (ERS) is crucial in the tumor microenvironment, often triggered by protein misfolding.
- Long non-coding RNAs (lncRNAs) are implicated in ERS response and lung adenocarcinoma (LUAD) progression, but their specific prognostic roles in LUAD are unclear.
Purpose Of The Study
- To identify ERS-associated lncRNAs with prognostic value in LUAD.
- To develop and validate a predictive model for LUAD patient survival based on ERS-related lncRNAs.
- To investigate the clinical implications and potential therapeutic targeting of these lncRNAs.
Main Methods
- Cox and least absolute shrinkage and selection operator (LASSO) regression analyses were used to identify prognostic ERS-related lncRNAs.
- A risk prediction model was constructed to classify LUAD patients into high-risk (HS) and low-risk (LS) groups.
- Comprehensive bioinformatic analyses, including functional enrichment and validation in clinical samples, were performed.
Main Results
- Nine ERS-related lncRNAs (five protective, four risk factors) were identified as independent prognostic indicators.
- The developed risk model effectively predicted overall survival and stratified LUAD patients.
- HS patients exhibited advanced-stage tumors, higher mutation burdens, impaired anti-tumor immunity, and reduced drug sensitivity compared to LS patients.
- Functional analysis linked these lncRNAs to cell migration, death, and immunity.
- Knockdown of the risk lncRNA RP11-295G20.2 reduced ERS and suppressed LUAD cell proliferation, invasion, and migration.
Conclusions
- A novel ERS-related lncRNA signature serves as a valuable biomarker for prognostic prediction in LUAD.
- The lncRNA RP11-295G20.2 is a potential therapeutic target for LUAD, as its modulation affects ERS and tumor progression.
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