Reference values of lymphocyte subsets from healthy Polish adults

Area of Science:

• Immunology
• Clinical Diagnostics
• Flow Cytometry

Background:

• Flow cytometry aids in diagnosing and monitoring immunodeficient patients.
• Clinical guidelines often focus on T, B, and NK cells, but reference values vary globally.
• Limited data exists for adult Polish populations regarding lymphocyte subsets.

Purpose of the Study:

• Determine reference values for T, B, and NK lymphocyte subsets in adult Poles.
• Analyze sex-based differences in lymphocyte subset percentages and counts.
• Investigate age-related correlations within specific lymphocyte subsets.

Main Methods:

• Utilized flow cytometry to analyze T, B, and NK cell subsets.
• Collected data from an adult Polish population.
• Stratified results by sex and age groups.

Main Results:

• Females exhibited higher percentages of CD45++, T helper cells, CD4/CD8 ratio, marginal zone-like B cells, class-switched B cells, and CD21low B cells.
• Males showed elevated percentages of naïve B lymphocytes, transitional B cells, and plasmablasts.
• Age correlated positively with double positive T, NKT, and CD21low B cells, and negatively with double negative T cells, marginal zone-like B cells, and plasmablasts.

Conclusions:

• Established sex- and age-specific reference ranges for lymphocyte subsets in adult Poles.
• Emphasized the importance of distinct reference ranges for accurate immunophenotyping.
• Findings support improved clinical decision-making for immunodeficient patients.