EDC and sulfo-NHS functionalized on PVC-g-PEGMA for streptokinase immobilization

In this work, streptokinase (SK) coenzyme was coupled onto functionalized graft copolymer poly(vinyl chloride)-g-poly(ethylene glycol)methacrylate (PEGMA) using the water soluble carbodiimide 1-ethyl-3-(3-dimethyl aminopropyl carbodiimide hydrochloride) and sulfo-N-Hydroxysulfo succinimide. Different graft values of PEGMA copolymers were prepared via preirradiation method in air by using ultraviolet light. SK was coupled onto graft copolymer and the enzyme activity was determined before and after immobilization. Surface morphology, grafting, and enzymatic activity were studied by using infrared spectroscopy, atomic force microscopy, and fluorescence microscopy. Results revealed that graft value of 0.15 provide optimum conditions to retain original SK activity, the enzyme distribution was 2.8 μg cm^-2 of copolymer surface and kinetic parameters, K _m and V _max were 0.14 μM and 29.8 μM min^-1, respectively.