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A Clinical Trial Assessing the Safety, Efficacy, and Delivery of Olive-Oil-Based Three-Chamber Bags for Parenteral Nutrition
Published on: September 20, 2019
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Hepatotoxicity assessment of innovative nutritional supplements based on olive-oil formulations enriched with natural
Sofia I Prodromou1, Fani Chatzopoulou2,3, Aikaterini Saiti1
1Laboratory of Pharmacology, School of Pharmacy, Aristotle University of Thessaloniki, Thessaloniki, Greece.
Frontiers in Nutrition
|May 30, 2024
Summary
Extra virgin olive oil and antioxidant formulations show minimal toxicity and benefit metabolic pathways in liver cells. These findings support their use in dietary supplements to combat oxidative stress and inflammation in diseases like Alzheimer's disease.
Area of Science:
- Nutritional Science
- Molecular Biology
- Pharmacology
Background:
- Oxidative stress and inflammation are implicated in the pathophysiology of various diseases, including Alzheimer's disease (AD).
- Nutritional interventions, particularly those involving natural antioxidants, are being explored for their potential therapeutic benefits.
Purpose of the Study:
- To assess extra virgin olive oil and olive fruit-based formulations with natural antioxidants as nutritional supplements.
- To evaluate the impact of these formulations on cell viability, proliferation, and hepatotoxicity in human hepatocellular carcinoma HepG2 cells.
- To investigate the effects on molecular pathways through gene expression profiling.
Main Methods:
- Cell viability and proliferation assays were performed on HepG2 cells exposed to the formulations.
- Hepatotoxicity was assessed using quantitative real-time PCR for gene expression profiling.
- Comparison of natural extracts versus chemical forms and different antioxidant delivery modes.
Main Results:
- The formulations exhibited minimal cellular toxicity in HepG2 cell cultures.
- A significant beneficial impact on metabolic molecular pathways was observed.
- Gene expression profiling provided insights into the molecular mechanisms of action.
Conclusions:
- Olive oil and antioxidant-rich formulations demonstrate potential as safe and effective nutritional supplements.
- These findings support innovative therapeutic strategies for managing oxidative stress and inflammation in AD and similar disorders.
- Advanced molecular methodologies are crucial for validating nutritional products and improving public health outcomes.
