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Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

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Development of a microcantilever-based biosensor for detecting Programmed Death Ligand 1.

Tajweed Neairat1, Mahmoud Al-Gawati1,2, Qura Tul Ain3

  • 1Department of Physics and Astronomy, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.

Saudi Pharmaceutical Journal : SPJ : the Official Publication of the Saudi Pharmaceutical Society
|May 30, 2024
PubMed
Summary

This study presents a novel microcantilever biosensor for detecting soluble Programmed Death Ligand 1 (sPD-L1), a key cancer biomarker. The biosensor offers a sensitive, specific, and efficient method for cancer diagnosis and immunotherapy monitoring.

Keywords:
AFMBiosensorCantilever-based sensorsProgram Death Ligand 1

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Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Cancer Biomarkers

Background:

  • Cancer diagnosis and immunotherapy monitoring require sensitive biomarker detection.
  • Programmed Death Ligand 1 (PD-L1) is a critical biomarker for cancer prognosis and therapy response.
  • Existing detection methods like ELISA have limitations in cost, time, and complexity.

Purpose of the Study:

  • To develop and validate a microcantilever-based biosensor for detecting soluble PD-L1 (sPD-L1).
  • To establish a sensitive, specific, and efficient detection method for PD-L1 in biological samples.

Main Methods:

  • A microcantilever biosensor functionalized with anti-PD-L1 antibodies was fabricated.
  • Atomic Force Microscopy (AFM) and contact angle measurements confirmed the sensing layer.
  • Detection principle relies on the shift in microcantilever resonance frequency upon sPD-L1 binding.

Main Results:

  • The biosensor demonstrated a linear response to sPD-L1 concentrations from 0.05 ng/ml to 500 ng/ml.
  • A low detection limit of approximately 10 pg/ml was achieved.
  • High specificity was observed even in complex biological matrices.

Conclusions:

  • The developed microcantilever biosensor is a promising tool for early cancer diagnosis.
  • This technology holds potential for monitoring cancer immunotherapy efficacy.
  • It offers a sensitive, specific, and efficient alternative to conventional detection methods.