Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Laminins are the Adhesive Proteins of Basal Lamina00:55

Laminins are the Adhesive Proteins of Basal Lamina

2.1K
Laminins are heterotrimeric proteins with high molecular mass found in the extracellular matrix. Each laminin molecule is composed of three chains, viz. alpha, beta, and gamma, coded by five, four, and three paralogous genes, respectively. Laminins are categories based on the compositions of the three chains.
In humans, the five forms of alpha chains are LAMA 1, LAMA 2, LAMA 3, LAMA 4, and LAMA 5. The four forms of beta chains are LAMB 1, LAMB 2, LAMB 3, and LAMB 4. The three forms of gamma...
2.1K
Mechanism of Lamellipodia Formation01:31

Mechanism of Lamellipodia Formation

2.5K
Cells migrating in response to external stimuli form lamellipodia, which are thin membrane protrusions supported by a mesh of linked, branched, or unbranched actin filaments. These actin filaments interact with myosin motor proteins, creating the dynamic actomyosin complex within the cytoskeleton. Contractility, or the ability to generate contractile stress, is inherent to the actomyosin complex. It helps cells detect the stiffness of the surrounding ECM and exert contractile force for...
2.5K
Mechanism of Filopodia Formation01:39

Mechanism of Filopodia Formation

2.3K
Filopodia are thin, actin-rich cellular protrusions that play an important role in many fundamental cellular functions. They vary in their occurrence, length, and positioning in different cell types, suggesting their diverse roles.
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
2.3K
Anchoring Junctions01:03

Anchoring Junctions

3.8K
Anchoring junctions are multiprotein complexes that help cells connect to other cells and the extracellular matrix. Anchoring junctions are present on the lateral and basal surfaces of cells, providing strong and flexible connections. Focal adhesions are often formed due to cell interactions with the ECM substrata, which initiate signal transduction via kinase cascades and other mechanisms. Together, they provide stability and tissue integrity. There are three types of anchoring junctions:...
3.8K
Selectins01:25

Selectins

3.3K
Cell adhesion is  an essential aspect of multicellularity. While stable cell interactions usually occur between cells of the same type, transient cell interactions occur between cells of different tissue types, such as between neutrophils and endothelial cells. Selectins are one class of cell adhesion molecules (CAMs) that bind carbohydrate ligands to form transient cell adhesion. They are rod-like proteins with a long extracellular part of variable length ending with the lectin domain,...
3.3K
Intracellular Signaling Affects Focal Adhesions01:17

Intracellular Signaling Affects Focal Adhesions

2.7K
Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
2.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Examination of Factors Affecting the Efficacy of Naldemedine Tosilate Tablets for Opioid-Induced Constipation].

Gan to kagaku ryoho. Cancer & chemotherapy·2026
Same author

Relationship between salivary inflammatory mediators and oral dryness during chemotherapy in patients with cancer: a cohort study.

Supportive care in cancer : official journal of the Multinational Association of Supportive Care in Cancer·2026
Same author

Intracellular GRP78-Directed Delivery of Rapamycin by Biomolecular Condensates of Hydra-Elastin-like Polypeptides.

Biomacromolecules·2026
Same author

Evaluation of LDV-Related Peptides as Cell-Adhesive Molecules Targeting α4β1 Integrin.

Biochemistry·2025
Same author

Development and validation of predictive model for emesis in cervical cancer patients receiving concurrent chemoradiotherapy based on multi-institutional retrospective study.

Scientific reports·2025
Same author

Restoring mitochondrial quantity and quality to reverse the Warburg effect and drive neuroblastoma differentiation.

Proceedings of the National Academy of Sciences of the United States of America·2025

Related Experiment Video

Updated: Jun 25, 2025

Generating a Fractal Microstructure of Laminin-111 to Signal to Cells
06:56

Generating a Fractal Microstructure of Laminin-111 to Signal to Cells

Published on: September 28, 2020

1.0K

Synergy between Laminin-Derived Elastin-like Polypeptides (LELPs) Optimizes Cell Spreading.

Anh T Truong1,2, Shin-Jae Lee3, Keisuke Hamada2

  • 1Department of Pharmacology and Pharmaceutical Sciences, Alfred E. Mann School of Pharmacy and Pharmaceutical Sciences, University of Southern California, Los Angeles, California 90089, United States.

Biomacromolecules
|May 30, 2024
PubMed
Summary

Researchers developed elastin-like polypeptides (ELPs) to create biomaterials that mimic the extracellular matrix (ECM). These novel ECM-mimetics enhance cell adhesion, spreading, and neurite outgrowth, showing promise for regenerative medicine.

More Related Videos

Production of Elastin-like Protein Hydrogels for Encapsulation and Immunostaining of Cells in 3D
11:46

Production of Elastin-like Protein Hydrogels for Encapsulation and Immunostaining of Cells in 3D

Published on: May 19, 2018

12.4K
Synthesis of Biocompatible Liquid Crystal Elastomer Foams as Cell Scaffolds for 3D Spatial Cell Cultures
13:38

Synthesis of Biocompatible Liquid Crystal Elastomer Foams as Cell Scaffolds for 3D Spatial Cell Cultures

Published on: April 11, 2017

9.4K

Related Experiment Videos

Last Updated: Jun 25, 2025

Generating a Fractal Microstructure of Laminin-111 to Signal to Cells
06:56

Generating a Fractal Microstructure of Laminin-111 to Signal to Cells

Published on: September 28, 2020

1.0K
Production of Elastin-like Protein Hydrogels for Encapsulation and Immunostaining of Cells in 3D
11:46

Production of Elastin-like Protein Hydrogels for Encapsulation and Immunostaining of Cells in 3D

Published on: May 19, 2018

12.4K
Synthesis of Biocompatible Liquid Crystal Elastomer Foams as Cell Scaffolds for 3D Spatial Cell Cultures
13:38

Synthesis of Biocompatible Liquid Crystal Elastomer Foams as Cell Scaffolds for 3D Spatial Cell Cultures

Published on: April 11, 2017

9.4K

Area of Science:

  • Biomaterials Science
  • Cell Biology
  • Regenerative Medicine

Background:

  • Laminins, key extracellular matrix (ECM) components, regulate cell behavior through various receptors.
  • Fragments of laminins are being explored as ECM-mimetics for cell-based therapies.
  • Elastin-like polypeptides (ELPs) offer a noncovalent method to immobilize bioactive peptides.

Purpose of the Study:

  • To generalize the use of ELPs for creating diverse ECM-mimetics from laminin-derived peptides.
  • To investigate the effects of specific laminin peptide-ELP fusions on cell morphology and behavior.
  • To explore synergistic effects of peptide mixtures and their potential in promoting neurite outgrowth.

Main Methods:

  • Fusion of four distinct laminin-derived peptides (A99, A2G80, AG73, EF1m) to elastin-like polypeptides (ELPs).
  • Assessment of cell adhesion, spreading, and F-actin formation on surfaces coated with these laminin-ELP conjugates.
  • Evaluation of neurite outgrowth in PC-12 cells cultured with the developed ECM-mimetics.

Main Results:

  • All tested laminin-ELP conjugates (LELPs) promoted cell spreading with distinct F-actin organization.
  • Equimolar mixtures of A99-LELP and A2G80-LELP exhibited synergistic effects on cell adhesion and spreading.
  • Three of the developed ECM-mimetics significantly promoted neurite outgrowth in PC-12 cells.

Conclusions:

  • ELPs provide a versatile platform for creating functional ECM-mimetics from laminin fragments.
  • These ECM-mimetics demonstrate potential for enhancing cell-material interactions.
  • The findings support the application of ELP-based ECM-mimetics in regenerative medicine, cell therapy, and tissue engineering.