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Tumor Pre-Targeting System Using Streptavidin-Expressing Bacteria.

Seong-Young Kwon1,2, Sung-Hwan You2,3, Jin Hee Im1,2

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Engineered bacteria express monomeric streptavidin (mSA) in tumors for targeted cancer therapy. This novel system enhances mSA expression and tumor visualization using biotinylated probes.

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Area of Science:

  • Oncology
  • Biotechnology
  • Molecular Imaging

Background:

  • Targeted cancer therapy requires specific tumor markers, which are often lacking.
  • Bacteria can selectively colonize solid tumors and deliver therapeutic payloads.
  • A pre-targeting system can improve the specificity and efficacy of tumor-directed agents.

Purpose of the Study:

  • To engineer bacteria for expressing monomeric streptavidin (mSA) within solid tumors.
  • To develop a novel tumor pre-targeting system using mSA and biotinylated imaging probes.
  • To assess the efficacy of this system for tumor visualization and targeting.

Main Methods:

  • Engineered E. coli to express mSA fused with maltose-binding protein under the pBAD promoter.
  • Administered engineered bacteria to mice with CT26 colon carcinoma tumors.
  • Induced mSA expression with L-arabinose and visualized tumor-associated mSA using a biotinylated fluorescent dye.
  • Quantified bacterial counts and confirmed mSA expression via Western blot.

Main Results:

  • The novel system achieved significantly higher mSA expression and sustained biotin binding compared to conventional methods.
  • Tumor tissues showed significantly stronger imaging signals in the mSA-expressing group.
  • Fluorescent signals in tumors were re-detectable after multiple L-arabinose inductions.
  • Bacterial counts in tumors were similar with or without L-arabinose induction.

Conclusions:

  • Successfully engineered tumor-targeting bacteria to express mSA within tumor tissues.
  • Demonstrated the potential of this novel pre-targeting system for cancer therapy.
  • This approach can be combined with biotinylated imaging probes or therapeutic agents for enhanced tumor targeting.