Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mismatch Repair01:20

Mismatch Repair

4.8K
Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
4.8K
Mutations01:39

Mutations

81.8K
Overview
81.8K
Cancer-Critical Genes II: Tumor Suppressor Genes01:05

Cancer-Critical Genes II: Tumor Suppressor Genes

7.4K
Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
7.4K
Abnormal Proliferation02:23

Abnormal Proliferation

4.5K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.5K
Loss of Tumor Suppressor Gene Functions01:12

Loss of Tumor Suppressor Gene Functions

4.8K
Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
4.8K
Comparing Copy Number Variations and SNPs02:26

Comparing Copy Number Variations and SNPs

17.7K
Sequencing of the human genome has opened up several best-kept secrets of the genome. Scientists have identified thousands of genome variations that exist within a population. These variations can be a single nucleotide or a larger chromosomal variation.
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...
17.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Whole genome sequencing in oesophageal adenocarcinoma unmasks potential precision therapies.

BMC cancer·2026
Same author

Enhanced measures of neoantigenicity capture unique tumor-immune interactions across primary melanoma subtypes.

Genome medicine·2026
Same author

Explainable machine learning identifies features and thresholds predictive of immunotherapy response.

Scientific reports·2026
Same author

Cancer genome standards for long-read sequencing using cancer cell line mixtures.

GigaScience·2026
Same author

Cancer genomic profiling predicts pathogenicity of <i>BRCA1</i> and <i>BRCA2</i> variants.

medRxiv : the preprint server for health sciences·2026
Same author

Multi-omics reveals key molecular and cellular features of advanced small cell lung cancers associated with distinct therapeutic opportunities.

Genome medicine·2026

Related Experiment Video

Updated: Jun 25, 2025

Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors
09:22

Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors

Published on: February 28, 2021

5.5K

Germline variants alter immune surveillance.

Nicola Waddell1, Venkateswar Addala1

  • 1Cancer Program, QIMR Berghofer Medical Research Institute, Brisbane, QLD, Australia.

Science (New York, N.Y.)
|May 30, 2024
PubMed
Summary

Germline-derived epitopes influence cancer development via the immune system. This immunoediting process is crucial for understanding tumor progression and potential therapeutic targets.

Area of Science:

  • Immunology
  • Oncology
  • Cancer Research

Background:

  • The immune system plays a critical role in cancer surveillance and elimination.
  • Tumor cells can evade immune detection through various mechanisms.
  • Germline-derived antigens present a unique aspect of the host-tumor interaction.

Purpose of the Study:

  • To investigate how germline-derived epitopes impact tumor development.
  • To elucidate the role of immunoediting in shaping the tumor microenvironment.
  • To understand the interplay between host immunity and cancer progression.

Main Methods:

  • Analysis of tumor samples for germline-derived epitope expression.
  • Immunohistochemistry and flow cytometry to assess immune cell infiltration.

More Related Videos

Screening for Functional Non-coding Genetic Variants Using Electrophoretic Mobility Shift Assay EMSA and DNA-affinity Precipitation Assay DAPA
11:35

Screening for Functional Non-coding Genetic Variants Using Electrophoretic Mobility Shift Assay EMSA and DNA-affinity Precipitation Assay DAPA

Published on: August 21, 2016

13.0K
Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease
09:34

Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease

Published on: April 4, 2018

33.7K

Related Experiment Videos

Last Updated: Jun 25, 2025

Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors
09:22

Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors

Published on: February 28, 2021

5.5K
Screening for Functional Non-coding Genetic Variants Using Electrophoretic Mobility Shift Assay EMSA and DNA-affinity Precipitation Assay DAPA
11:35

Screening for Functional Non-coding Genetic Variants Using Electrophoretic Mobility Shift Assay EMSA and DNA-affinity Precipitation Assay DAPA

Published on: August 21, 2016

13.0K
Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease
09:34

Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease

Published on: April 4, 2018

33.7K
  • In vivo studies using mouse models to track tumor growth and immune responses.

    • Main Results:

    • Germline-derived epitopes were identified on tumor cells, influencing immune recognition.
    • The presence of these epitopes correlated with specific immune cell profiles within the tumor microenvironment.
    • Immunoediting processes were modulated by the expression of germline-derived epitopes, affecting tumor growth rates.

    Conclusions:

    • Germline-derived epitopes are significant modulators of tumor development.
    • Immunoediting, influenced by germline epitopes, is a key mechanism in cancer progression.
    • Targeting germline-derived epitopes may offer novel strategies for cancer immunotherapy.