Fluorescence polarization assay by flow cytometry

Area of Science:

• Cellular immunology
• Biophysical techniques

Background:

• Fluorescence polarization (FP) is a sensitive biophysical technique.
• FP can detect molecular changes within cells.

Purpose of the Study:

• Review the principles of fluorescence polarization.
• Discuss its measurement using flow cytometry.
• Highlight applications in cellular immunology.

Main Methods:

• Fluorescence polarization measurements on cell suspensions.
• Utilizing a commercially available flow cytometer.

Main Results:

• FP is highly sensitive for detecting subtle cellular changes.
• Early changes after lymphocyte activation are detectable.
• Malignant transformation can be identified.

Conclusions:

• Fluorescence polarization is a powerful tool in cellular immunology.
• Flow cytometry enables practical FP measurements.
• FP assays offer significant potential for diagnostics and research.