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Updated: Jun 25, 2025

Imaging and Analysis for Quantifying Maize (Zea mays) Abiotic Stress Phenotypes
Published on: March 28, 2025
Early detection of nicosulfuron toxicity and physiological prediction in maize using multi-branch deep learning
Tianpu Xiao1, Li Yang1, Dongxing Zhang1
1College of Engineering, China Agricultural University, Beijing 100083, China; The Soil-Machine-Plant key laboratory of the Ministry of Agriculture of China, Beijing 100083, China.
Abstract:
The misuse of herbicides in fields can cause severe toxicity in maize, resulting in significant reductions in both yield and quality. Therefore, it is crucial to develop early and efficient methods for assessing herbicide toxicity, protecting maize production, and maintaining the field environment. In this study, we utilized maize crops treated with the widely used nicosulfuron herbicide and their hyperspectral images to develop the HerbiNet model. After 4 d of nicosulfuron treatment, the model achieved an accuracy of 91.37 % in predicting toxicity levels, with correlation coefficient R² values of 0.82 and 0.73 for soil plant analysis development (SPAD) and water content, respectively. Additionally, the model exhibited higher generalizability across datasets from different years and seasons, which significantly surpassed support vector machines, AlexNet, and partial least squares regression models. A lightweight model, HerbiNet-Lite, exhibited significantly low complexity using 18 spectral wavelengths. After 4 d of nicosulfuron treatment, the HerbiNet-Lite model achieved an accuracy of 87.93 % for toxicity prediction and R² values of 0.80 and 0.71 for SPAD and water content, respectively, while significantly reducing overfitting. Overall, this study provides an innovative approach for the early and accurate detection of nicosulfuron toxicity within maize fields.
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