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Related Experiment Videos

Human monocyte recognition of complement-coated lymphoblastoid cells.

S Armstrong, P McDermott, A D Schreiber

    Journal of Leukocyte Biology
    |February 1, 1985
    PubMed
    Summary

    Monocytes recognize leukemia cells via complement, specifically requiring significant C3 deposition. This complement-mediated recognition, while similar to non-malignant cells, may be inefficient for tumor cells.

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    Area of Science:

    • Immunology
    • Cell Biology

    Background:

    • Macrophages are crucial for foreign cell detection.
    • Mechanisms of macrophage recognition of malignant cells are not fully understood.

    Purpose of the Study:

    • Investigate initial monocyte recognition of tumor cells.
    • Elucidate the role of complement in monocyte-leukemia cell interactions.

    Main Methods:

    • Used human monocytes and lymphoblastoid cell (LC) lines from acute lymphocytic leukemia.
    • Assessed monocyte binding to IgM-coated LC with varying complement components.
    • Quantified C3 deposition on LC.

    Main Results:

    • Monocyte binding to IgM-coated LC required complement, with C3 being essential.

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  • Complement activation led to C3 consumption and deposition on LC.
  • High C3 deposition (approx. 4.0 x 10^5 molecules/LC) was necessary for monocyte recognition.

    • Conclusions:

    • Monocytes recognize leukemia cells via complement pathways similar to non-malignant cells.
    • Inefficiency in complement-mediated recognition may arise from the substantial C3 amounts required for IgM-coated LC recognition.