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Correlation between Matrix Metalloproteinase Presence and Caries Surface Appearance.
Lamis El Sharkasi1, Lynne Bingle1, Nicolas Martin1
1School of Clinical Dentistry, The University of Sheffield, Sheffield, UK.
Caries Research
|May 30, 2024
Summary
Matrix metalloproteinases (MMPs) increase significantly with advanced dental caries (ICDAS 4-6). This study correlates MMP2 and MMP9 expression with caries severity in primary teeth, supporting minimally invasive treatment approaches.
Area of Science:
- Biochemistry
- Dental Research
- Immunohistochemistry
Background:
- Cariogenic acids demineralize tooth structure, exposing dentin matrix.
- Matrix metalloproteinases (MMPs) are key regulators of extracellular matrix turnover and collagen degradation in caries progression.
Purpose of the Study:
- To investigate the expression of MMP2 and MMP9 in primary human teeth at various caries stages.
- To correlate MMP expression with caries severity diagnosed using the International Caries Detection and Assessment System (ICDAS).
Main Methods:
- Immunohistochemistry was used to detect MMP2 and MMP9 expression in 24 extracted teeth.
- Teeth were clinically diagnosed using ICDAS and stained with anti-MMP antibodies.
- Collagen degradation was assessed via hydroxyproline assay; images analyzed using ImageJ.
Main Results:
- MMP expression was low in early caries (ICDAS 1-3) but significantly increased in advanced caries (ICDAS 4-6).
- A strong positive correlation was found between ICDAS scores and MMP2 (r=0.86) and MMP9 (r=0.82) expression.
- Statistical analysis confirmed significant differences in MMP levels across caries severity.
Conclusions:
- ICDAS effectively assesses caries severity and correlates with MMP presence.
- Findings support the minimally invasive concept in modern caries management by understanding MMP involvement.
