Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

  1. Home
  3. Research Domains
  5. Biomedical And Clinical Sciences
  7. Oncology And Carcinogenesis
  9. Predictive And Prognostic Markers
  11. Controlled Delivery Of Procyanidin Through Magnesium Oxide Nanoparticles (mgo Nps) To Improve The Activity And Mineralization Of Osteoblasts Under Oxidative Stressin Vitro

Controlled delivery of procyanidin through magnesium oxide nanoparticles (MgO NPs) to improve the activity and mineralization of osteoblasts under oxidative stressin vitro

Shihua Lu1, Yingli Zhu2, Jianfan Lin2

  • 1Guangxi Food and Drug Evaluation & Inspection Center, Food and Drug Administration of Guangxi, No. 32 Yunjing Road, Nanning, Guangxi, People's Republic of China.

Biomedical Materials (Bristol, England)
|May 30, 2024

Related Experiment Videos

Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells
06:47

Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells

Published on: May 4, 2018

9.4K
Evaluation of Antimicrobial Activities of Nanoparticles and Nanostructured Surfaces In Vitro
11:52

Evaluation of Antimicrobial Activities of Nanoparticles and Nanostructured Surfaces In Vitro

Published on: April 21, 2023

3.1K
Preparation Of Gushukang GSK Granules for In Vivo and In Vitro Experiments
06:16

Preparation Of Gushukang GSK Granules for In Vivo and In Vitro Experiments

Published on: May 9, 2019

7.6K

View abstract on PubMed

Summary
This summary is machine-generated.

Magnesium oxide nanoparticles loaded with procyanidins (MgO-PC NPs) effectively combat oxidative stress in osteoporosis models. These nanoparticles enhance osteoblast activity and bone mineralization, offering a promising therapeutic strategy for bone loss conditions.

Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Osteoporosis Research

Background:

  • Osteoporosis (OP) is characterized by excessive reactive oxygen species (ROS) that impair osteoblast function and accelerate bone resorption.
  • Procyanidins (PC) possess antioxidant properties but suffer from poor bioavailability, limiting their therapeutic application.
  • Developing effective delivery systems for antioxidants is crucial for managing oxidative stress in OP.

Purpose of the Study:

  • To investigate the therapeutic potential of procyanidin-loaded magnesium oxide nanoparticles (MgO-PC NPs) for osteoporosis.
  • To evaluate the effect of MgO-PC NPs on osteogenesis and mineralization of osteoblasts under oxidative stress.
  • To assess the antioxidant capacity and controlled release of procyanidins from MgO-PC NPs.

Main Methods:

Keywords:
magnesium oxide nanoparticlesmineralizationosteoblastsoxidative stress

Related Experiment Videos

Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells
06:47

Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells

Published on: May 4, 2018

9.4K
Evaluation of Antimicrobial Activities of Nanoparticles and Nanostructured Surfaces In Vitro
11:52

Evaluation of Antimicrobial Activities of Nanoparticles and Nanostructured Surfaces In Vitro

Published on: April 21, 2023

3.1K
Preparation Of Gushukang GSK Granules for In Vivo and In Vitro Experiments
06:16

Preparation Of Gushukang GSK Granules for In Vivo and In Vitro Experiments

Published on: May 9, 2019

7.6K
  • Procyanidins (PC) were loaded onto magnesium oxide nanoparticles (MgO NPs) to form MgO-PC NPs.
  • Characterization of MgO-PC NPs using techniques including TEM, EDS, DLS, and FTIR.
  • Assays for ROS production, cell viability (CCK-8), osteogenic markers (ALP, Alizarin Red), and gene expression (qRT-PCR) in H2O2-stimulated osteoblasts.

Main Results:

  • Successfully synthesized MgO-PC NPs with a diameter of approximately 144 nm and negative potential, demonstrating sustained PC release for at least 16 days.
  • MgO-PC NPs effectively scavenged ROS, significantly enhanced cell viability, and promoted osteoblast activity under oxidative stress.
  • The treatment with MgO-PC NPs reversed the negative effects of oxidative stress on osteogenesis and mineralization markers in osteoblasts.

Conclusions:

  • MgO-PC NPs represent a promising nanocarrier system for procyanidins, enhancing their bioavailability and antioxidant efficacy.
  • The controlled release of PC from MgO-PC NPs effectively mitigates ROS-induced damage in osteoblasts.
  • MgO-PC NPs demonstrate significant potential as a therapeutic strategy for osteoporosis by promoting bone formation and mineralization.
procyanidin