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A 3D Bioprinted Cortical Organoid Platform for Modeling Human Brain Development.

Melissa A Cadena1,2, Anson Sing2, Kylie Taylor2

  • 1Department of Biomedical Engineering, Emory University School of Medicine and Georgia Institute of Technology, Atlanta, GA, 30322, USA.

Advanced Healthcare Materials
|May 30, 2024
PubMed
Summary

3D bioprinting creates tunable scaffolds for brain organoid development. This platform enables better control over the extracellular environment, supporting healthier organoid growth and vascularization for disease modeling.

Keywords:
3D bioprintingbrain organoidsextracellular matrixinduced pluripotent stem cellsvasculature

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Area of Science:

  • Developmental Biology
  • Tissue Engineering
  • Neuroscience

Background:

  • Organoids, particularly brain organoids, are valuable for studying neurodevelopment and diseases.
  • Current brain organoid cultures lack precise control over their extracellular environment.
  • 3D self-organization of stem cells into organoids offers new research opportunities.

Purpose of the Study:

  • To develop a 3D bioprinting platform for controlled organoid development and patterning.
  • To create a scaffold supporting co-culture of organoids and vascular cells.
  • To establish a more accurate model for human brain development and disease.

Main Methods:

  • Utilized 3D bioprinting to fabricate tunable and reproducible scaffolds.
  • Incorporated interconnected endothelialized channels for vascular cell co-culture.
  • Assessed scaffold fidelity, mechanical properties, and organoid development (growth, viability, cytoarchitecture, proliferation, transcriptomics).

Main Results:

  • Bioprinted scaffolds demonstrated high printing fidelity and mechanical properties similar to the developing human brain.
  • Organoids cultured long-term in scaffolds showed healthy growth, viability, and expected neuroectodermal differentiation.
  • Endothelial cells successfully migrated into and infiltrated the embedded organoids within the printed channels.

Conclusions:

  • 3D bioprinting provides a tunable platform for precise control over organoid development.
  • This approach supports the creation of more complex and accurate models of human brain development.
  • The platform facilitates the co-culture of organoids with vascular networks for enhanced disease modeling.