Transgenic female mice producing trans 10, cis 12-conjugated linoleic acid present excessive prostaglandin E2, adrenaline, corticosterone, glucagon, and FGF21

Yu Rao ^1,2, Lu-Wen Liang ¹, Mei-Juan Li ³

⁰Institute of Comparative Medicine, College of Veterinary Medicine, Yangzhou University, Yangzhou, 225009, China.

Scientific Reports +

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May 30, 2024

View abstract on PubMed

Summary

Dietary trans 10, cis 12-conjugated linoleic acid (t10c12-CLA) did not reduce fat in female mice but enhanced lipolysis and thermogenesis. It also caused hepatic steatosis and hypertriglyceridemia, suggesting complex metabolic effects.

Area Of Science

Metabolic research
Endocrinology
Obesity research

Background

Dietary trans 10, cis 12-conjugated linoleic acid (t10c12-CLA) is explored for anti-obesity effects.
Previous studies established transgenic mice producing endogenous t10c12-CLA in males.

Purpose Of The Study

To investigate the sex-specific impact of t10c12-CLA on lipid metabolism.
To assess adiposity and metabolic profiles in female transgenic mice (Pai mice) producing t10c12-CLA.

Main Methods

Studied female Pai mice with dose-dependent foreign gene expression and t10c12-CLA levels.
Analyzed adiposity, lipolysis, thermogenesis, hepatic steatosis, and hypertriglyceridemia.
Measured hormone and inflammatory factor levels, including prostaglandin E2, adrenaline, corticosterone, glucagon, and FGF21.

Main Results

Pai mice showed no fat reduction but increased lipolysis and thermogenesis.
Hepatic steatosis and hypertriglyceridemia were observed, linked to altered lipid/glucose metabolism gene expression.
Elevated prostaglandin E2, adrenaline, corticosterone, glucagon, and inflammatory factors were noted, reducing heat release and oxygen consumption.
Fibroblast growth factor 21 overproduction in monoallelic Pai/wt mice indicated sensitivity to low t10c12-CLA doses.

Conclusions

Chronic t10c12-CLA affects female lipid metabolism and overall health.
The compound induces complex hormonal and inflammatory responses, impacting thermogenesis and energy expenditure.
Results highlight the need for further research into sex-specific effects and therapeutic potential of t10c12-CLA.

Keywords:

Adipose Conjugated linoleic acid Energy expenditure Inflammation Steatosis

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